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Literature summary extracted from
- The composition of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Does a unifying model exist for the complexes from gram-negative bacteria? (1984), Eur. J. Biochem., 142, 541-549.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.104 | 94000 | - |
2 * 94000, SDS-PAGE | Azotobacter vinelandii |
| 1.2.1.104 | 190000 | - |
sedimentation equilibrium centrifugation | Azotobacter vinelandii |
| 1.8.1.4 | 56000 | - |
2 * 56000, SDS-PAGE | Azotobacter vinelandii |
| 1.8.1.4 | 100000 | - |
equilibrium sedimentation | Azotobacter vinelandii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.104 | Azotobacter vinelandii | - |
- |
- |
| 1.8.1.4 | Azotobacter vinelandii | - |
dihydrolipoamide dehydrogenase E3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.104 | pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 | Azotobacter vinelandii |
| 1.8.1.4 | from pyruvate dehydrogenase complex | Azotobacter vinelandii |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.2.1.104 | reconstitution of pyruvate dehydrogenase complex | Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.104 | 15 | 19 | - |
Azotobacter vinelandii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.2.1.104 | liquid N2, 50 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 0.05 mM phenylmethanesulfonylfluoride, 20-40 mg/ml protein | Azotobacter vinelandii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.4 | dihydrolipoamide + NAD+ | - |
Azotobacter vinelandii | lipoamide + NADH | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.1.104 | dimer | 2 * 94000, SDS-PAGE | Azotobacter vinelandii |
| 1.8.1.4 | dimer | 2 * 56000, SDS-PAGE | Azotobacter vinelandii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.4 | NAD+ | - |
Azotobacter vinelandii | |
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Release 2023.1 (January 2023)
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