Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yeaman, S.J.
    The mammalian 2-oxoacid dehydrogenases: a complex family (1986), Trends Biochem. Sci., 11, 293-296.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.12 sensitive to proteolysis Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.104 phosphorylation
-
 Bos taurus
1.2.1.104 phosphorylation
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.105 mitochondrion
-
 Sus scrofa 5739
-
2.3.1.12 mitochondrion
-
 Bos taurus 5739
-
2.3.1.12 mitochondrion pyruvate dehydrogenase complex bound to inner mitochondrial membrane Mammalia 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.105 Ca2+ activates Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.104 additional information
-
-
 Sus scrofa
1.2.1.104 additional information
-
 MW of native complex isolated from kidney and heart: 7000000 Da and 8500000 Da respectively Bos taurus
1.2.1.104 36000
-
 alpha2,beta2, 2 * 41000 + 2 * 36000 Bos taurus
1.2.1.104 36000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 41000
-
 alpha2,beta2, 2 * 41000 + 2 * 36000 Bos taurus
1.2.1.104 41000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 50000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 52000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 55000
-
 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
2.3.1.12 48000
-
 24 * 48000 Sus scrofa
2.3.1.12 52000
-
 60 * 52000 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.105 2-oxoglutarate + lipoamide Sus scrofa enzyme of the TCA cycle S-succinyldihydrolipoamide + CO2
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA Mammalia
-
 S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA Sus scrofa
-
 S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA Bos taurus
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Bos taurus
-
 overview
-
1.2.1.104 Sus scrofa
-
 overview
-
1.2.1.105 Sus scrofa
-
-
-
2.3.1.12 Bos taurus
-
-
-
2.3.1.12 Mammalia
-
-
-
2.3.1.12 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.104 side-chain modification
-
 Bos taurus
1.2.1.104 side-chain modification inactivated by phosphorylation Sus scrofa
2.3.1.12 lipoprotein
-
 Mammalia
2.3.1.12 lipoprotein
-
 Sus scrofa
2.3.1.12 lipoprotein
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.105 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 2-oxoglutarate dehydrogenase complex consists of 3 enzymes: E1 (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), E2 (dihydrolipoyl transsuccinylase, EC 2.3.1.61), E3 (dihydrolipoyl dehydrogenase, EC 1.8.1.4) Sus scrofa
a
1.2.1.105 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.105 heart
-
 Sus scrofa
-
2.3.1.12 heart
-
 Sus scrofa
-
2.3.1.12 heart
-
 Bos taurus
-
2.3.1.12 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.105 2-oxoglutarate + lipoamide
-
 Sus scrofa S-succinyldihydrolipoamide + CO2
-
 ?
1.2.1.105 2-oxoglutarate + lipoamide enzyme of the TCA cycle Sus scrofa S-succinyldihydrolipoamide + CO2
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Mammalia S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Sus scrofa S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Bos taurus S-acetyldihydrolipoamide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.104 oligomer
-
 Sus scrofa
1.2.1.104 oligomer 60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex Bos taurus
1.2.1.104 tetramer alpha2,beta2, 2 * 41000 + 2 * 36000 Bos taurus
1.2.1.105 More structure of the 2-oxoglutarate dehydrogenase complex: E1 (EC 1.2.4.2): 12 subunits per complex (each with 113000 Da), E2 (EC 2.3.1.61): 24 subunits per complex (each with 48000 Da), E3 (EC 1.8.1.4): 12 subunits per complex (each with 55000 Da) Sus scrofa
2.3.1.12 polymer 24 * 48000 Sus scrofa
2.3.1.12 polymer 60 * 52000 Bos taurus
2.3.1.12 polymer stoichiometry of pyruvate dehydrogenase complexes Mammalia

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.105 thiamine diphosphate covalently bound to the enzyme Sus scrofa