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Literature summary extracted from

  • Lindahl, R.; Evces, S.
    Rat liver aldehyde dehydrogenase. I. Isolation and characterization of four high Km normal liver isozymes (1984), J. Biol. Chem., 259, 11986-11990.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Chloral hydrate inhibition of isoenzyme I and II from mitochondria and isoenzyme II from microsomes, no inhibition of isoenzyme I from microsomes Rattus norvegicus
1.2.1.3 Disulfiram inhibits activity of mitochondrial isoenzyme I with NAD+ and activity of isoenzyme I and II from microsomes and mitochondria with NADP+ Rattus norvegicus
1.2.1.3 NAD+ 0.1 mM, significant inhibition of esterase activity Rattus norvegicus
1.2.1.3 p-hydroxymercuribenzoate 0.05 mM, potent inhibition of isoenzyme I from microsomes and mitochondria, isoenzyme II from microsomes and mitochondria is less sensitive Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.022
-
 NAD+ reaction with propanal, enzyme II from mitochondria Rattus norvegicus
1.2.1.3 0.036
-
 NAD+ reaction with propanal, enzyme I from mitochondria Rattus norvegicus
1.2.1.3 0.095
-
 NAD+ reaction with propanal, enzyme II from microsomes Rattus norvegicus
1.2.1.3 0.097
-
 NAD+ reaction with propanal, enzyme I from microsomes Rattus norvegicus
1.2.1.3 0.15
-
 propanal enzyme I and II from mitochondria Rattus norvegicus
1.2.1.3 0.27
-
 NADP+ reaction with benzaldehyde, enzyme II from microsomes Rattus norvegicus
1.2.1.3 0.44
-
 NADP+ reaction with benzaldehyde, enzyme II from mitochondria Rattus norvegicus
1.2.1.3 0.44
-
 NADP+ reaction with benzaldehyde, enzyme I from microsomes Rattus norvegicus
1.2.1.3 0.91
-
 NADP+ reaction with benzaldehyde, enzyme I from mitochondria Rattus norvegicus
1.2.1.3 1.6
-
 benzaldehyde enzyme I from mitochondria Rattus norvegicus
1.2.1.3 1.7
-
 propanal enzyme II from microsome Rattus norvegicus
1.2.1.3 2.4
-
 acetaldehyde enzyme I from microsome Rattus norvegicus
1.2.1.3 3
-
 benzaldehyde enzyme II from microsomes Rattus norvegicus
1.2.1.3 5.9
-
 benzaldehyde enzyme I from microsomes Rattus norvegicus
1.2.1.3 50
-
 benzaldehyde enzyme II from mitochondria Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 microsome isoenzyme I and II Rattus norvegicus
-
-
1.2.1.3 mitochondrion isoenzyme I and II Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Mg2+ 0.5 mM, slight activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 54000
-
 2 * 54000, SDS-PAGE Rattus norvegicus
1.2.1.3 170000
-
 mitochondrial isoenzyme I, gel filtration Rattus norvegicus
1.2.1.3 250000
-
 mitochondrial isoenzyme II, microsomal isoenzyme I and II, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.2.1.3 -20°C, all isoenzymes are stable for up to 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus acetate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O
-
 Rattus norvegicus benzoate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NADP+
-
 Rattus norvegicus benzoate + NADPH
-
 ?
1.2.1.3 p-nitrophenyl acetate + H2O esterase activity of the various isoenzymes is less than 7% of the activity with propanal and NAD+ Rattus norvegicus p-nitrophenol + acetate
-
 ?
1.2.1.3 phenylacetaldehyde + NAD+ + H2O
-
 Rattus norvegicus phenylacetate + NADH + H+
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Rattus norvegicus propionate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 dimer 2 * 54000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.3 55
-
 5 min, microsomal isoenzymes and mitochondrial isoenzyme I, more than 90% loss of activity. Mitochondrial isoenzyme II retains 20% of its original activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8.8
-
 microsomal isoenzyme I and II and mitochondrial isoenzymes with NADP+ Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+ all isoenzymes prefer NAD+ over NADP+ Rattus norvegicus
1.2.1.3 NADP+ all isoenzymes prefer NAD+ over NADP+ Rattus norvegicus