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Literature summary extracted from

  • Wang, X.; Mann, C.J.; Bai, Y.; Ni, L.; Weiner, H.
    Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae (1998), J. Bacteriol., 180, 822-830.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.5 expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.5 ZnCl2 50% inhibition at 0.035 mM, enzyme form ALDH1 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.5 0.01
-
 acetaldehyde reaction with NADP+, enzyme form ALDH2 Saccharomyces cerevisiae
1.2.1.5 0.013
-
 propanal reaction with NAD+, enzyme form ALDH2 Saccharomyces cerevisiae
1.2.1.5 0.017
-
 propanal reaction with NADP+, enzyme form ALDH2 Saccharomyces cerevisiae
1.2.1.5 0.024
-
 acetaldehyde reaction with NADP+, enzyme form ALDH1 Saccharomyces cerevisiae
1.2.1.5 0.03
-
 propanal reaction with NADP+, enzyme form ALDH1 Saccharomyces cerevisiae
1.2.1.5 0.04
-
 NADP+ reaction with acetaldehyde, enzyme form ALDH1 Saccharomyces cerevisiae
1.2.1.5 0.058
-
 acetaldehyde reaction with NADP+, enzyme form ALDH5 Saccharomyces cerevisiae
1.2.1.5 0.099
-
 NADP+ reaction with propanal, enzyme form ALDH1 Saccharomyces cerevisiae
1.2.1.5 0.39
-
 propanal reaction with NADP+, enzyme form ALDH5 Saccharomyces cerevisiae
1.2.1.5 0.447
-
 NADP+ reaction with propanal, enzyme form ALDH2 Saccharomyces cerevisiae
1.2.1.5 0.64
-
 NADP+ reaction with acetaldehyde, enzyme form ALDH5 Saccharomyces cerevisiae
1.2.1.5 0.7
-
 propanal reaction with NAD+, enzyme form ALDH1 Saccharomyces cerevisiae
1.2.1.5 0.83
-
 propanal reaction with NAD+, enzyme form ALDH5 Saccharomyces cerevisiae
1.2.1.5 1.1
-
 NAD+ reaction with propanal, enzyme form ALDH2 Saccharomyces cerevisiae
1.2.1.5 1.4
-
 NADP+ reaction with acetaldehyde, enzyme form ALDH2 Saccharomyces cerevisiae
1.2.1.5 3.47
-
 NADP+ reaction with propanal, enzyme form ALDH5 Saccharomyces cerevisiae
1.2.1.5 6.43
-
 NAD+ reaction with propanal, enzyme form ALDH5 Saccharomyces cerevisiae
1.2.1.5 17.4
-
 NAD+ reaction with propanal, enzyme form ALDH1 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.5 cytosol enzyme form ALDH1 Saccharomyces cerevisiae 5829
-
1.2.1.5 mitochondrion enzyme form ALDH5 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.5 CaCl2 30 mM, 3.8fold stimulation of activity of ALDH1 Saccharomyces cerevisiae
1.2.1.5 KCl 120 mM: 40fold increase of activity of ALDH5 with NAD+. 16 mM: 20fold increase of activity of enzyme ALDH5 with NADP+. No stimulation of the activity of enzyme form ALDH1 Saccharomyces cerevisiae
1.2.1.5 Mg2+ 20 mM MgCl2, 2.8fold stimulation of activity of ALDH1 Saccharomyces cerevisiae
1.2.1.5 MnCl2 2 mM, 2.1fold stimulation of activity of ALDH1 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.5 53904
-
 x * 53904, enzyme form ALDH5, mass spectrometric analysis Saccharomyces cerevisiae
1.2.1.5 54570
-
 x * 54570, enzyme form ALDH1, mass spectrometric analysis Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.5 Saccharomyces cerevisiae
-
 ALDH1, ALDH2 and ALDH5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.5
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.5 acetaldehyde + NAD+ + H2O
-
 Saccharomyces cerevisiae acetate + NADH + H+
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O ALDH2 is very active, low activity with ALDH1 and ALDH5 Saccharomyces cerevisiae benzoate + NADH + H+
-
 ?
1.2.1.5 propanal + NAD+ + H2O
-
 Saccharomyces cerevisiae propionate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.5 ? x * 53904, enzyme form ALDH5, mass spectrometric analysis Saccharomyces cerevisiae
1.2.1.5 ? x * 54570, enzyme form ALDH1, mass spectrometric analysis Saccharomyces cerevisiae