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Literature summary extracted from

  • Ryzlak, M.T.; Pietruszko, R.
    Purification and characterization of aldehyde dehydrogenase from human brain (1987), Arch. Biochem. Biophys., 255, 409-418.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 acetaldehyde 0.02 mM, substrate inhibition of isoenzyme I, no inhibition of isoenzyme II and III Homo sapiens
1.2.1.3 Disulfiram inhibition of isoenzyme III, isoenzyme I is extremely sensitive, isoenzyme II is totally insensitive Homo sapiens
1.2.1.3 Mg2+ isoenzyme III Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0005
-
 3,4-dihydroxyphenyl acetaldehyde isoenzyme II Homo sapiens
1.2.1.3 0.0007
-
 indole 3-acetaldehyde isoenzyme I Homo sapiens
1.2.1.3 0.001
-
 indole 3-acetaldehyde isoenzyme III Homo sapiens
1.2.1.3 0.003
-
 3,4-dihydroxyphenyl acetaldehyde isoenzyme III Homo sapiens
1.2.1.3 0.004
-
 propanal isoenzyme III Homo sapiens
1.2.1.3 0.005
-
 glycolaldehyde isoenzyme I Homo sapiens
1.2.1.3 0.021
-
 glycolaldehyde isoenzyme II Homo sapiens
1.2.1.3 0.048
-
 acetaldehyde isoenzyme III Homo sapiens
1.2.1.3 0.11
-
 glycolaldehyde isoenzyme III Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 cytoplasm isoenzyme III Homo sapiens 5737
-
1.2.1.3 mitochondrion isoenzyme I and II Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Mg2+ activation of isoenzyme I and II Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 54000
-
 4 * 54000, isoenzyme I, II and III, SDS-PAGE Homo sapiens
1.2.1.3 208000 210000 isoenzyme I, non-denaturing PAGE Homo sapiens
1.2.1.3 216000
-
 isoenzyme II, non-denaturing PAGE Homo sapiens
1.2.1.3 220000
-
 isoenzyme III, gel filtration Homo sapiens
1.2.1.3 236000
-
 isoenzyme III, non-denaturing PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 0.29
-
 isoenzyme I Homo sapiens
1.2.1.3 0.445
-
 isoenzyme III Homo sapiens
1.2.1.3 3
-
 isoenzyme II Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
-
 Homo sapiens 3,4-dihydroxyphenylacetate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
1.2.1.3 glycolaldehyde + NAD+ + H2O
-
 Homo sapiens glycolate + NADH
-
 ?
1.2.1.3 indole-3-acetaldehyde + NAD+ + H2O
-
 Homo sapiens indole-3-acetate + NADH + H+
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 tetramer 4 * 54000, isoenzyme I, II and III, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8
-
 reaction with indole 3-acetaldehyde, isoenzyme III Homo sapiens
1.2.1.3 8.5
-
 reaction with acetaldehyde and indole 3-acetaldehyde, isoenzyme II Homo sapiens
1.2.1.3 9
-
 reaction with acetaldehyde, isoenzyme III Homo sapiens
1.2.1.3 9
-
 reaction with indole 3-acetaldehyde, isoenzyme I Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Homo sapiens