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Literature summary extracted from

  • Kato, M.; Miura, Y.; Kettoku, M.; Shindo, K.; Iwamatsu, A.; Kobayashi, M.
    Purification and characterization of new trehalose-producing enzymes isolated from the hyperthermophilic archae, Sulfolobus sulfataricus KM1 (1996), Biosci. Biotechnol. Biochem., 60, 546-550.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.15 76000
-
 x * 76000, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.15 additional information Saccharolobus solfataricus enzyme is involved in the pathway of trehalose synthesis from starch ?
-
 ?
5.4.99.15 additional information Saccharolobus solfataricus KM1 enzyme is involved in the pathway of trehalose synthesis from starch ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.15 Saccharolobus solfataricus
-
 KM1
-
5.4.99.15 Saccharolobus solfataricus KM1
-
 KM1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.15
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.15 192
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.15 Maltopentaose
-
 Saccharolobus solfataricus alpha-Maltotriosyl trehalose
-
 ?
5.4.99.15 Maltopentaose
-
 Saccharolobus solfataricus KM1 alpha-Maltotriosyl trehalose
-
 ?
5.4.99.15 additional information enzyme is involved in the pathway of trehalose synthesis from starch Saccharolobus solfataricus ?
-
 ?
5.4.99.15 additional information enzyme is involved in the pathway of trehalose synthesis from starch Saccharolobus solfataricus KM1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.15 ? x * 76000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.15 70 80
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.15 85
-
 6 h, 9% loss of activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.15 5 6
-
 Saccharolobus solfataricus