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Literature summary extracted from

  • Li, Z.; Meighen, E.A.
    The turnover of bacterial luciferase is limited by a slow decomposition of the ternary enzyme-product complex of luciferase, FMN, and fatty acid (1994), J. Biol. Chem., 269, 6640-6644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 expression in Escherichia coli Photorhabdus luminescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.3 0.00015
-
 FMNH alpha subunit Photorhabdus luminescens
1.14.14.3 0.00058
-
 FMNH beta subunit Photorhabdus luminescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 decanal + FMNH + O2 Photorhabdus luminescens
-
 decanoic acid + FMN + H2O + light
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Photorhabdus luminescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 decanal + FMNH + O2
-
 Photorhabdus luminescens decanoic acid + FMN + H2O + light
-
 ir
1.14.14.3 dodecanal + FMNH + O2
-
 Photorhabdus luminescens dodecanoic acid + FMN + H2O + light
-
 ir
1.14.14.3 myristic aldehyde + FMNH + O2
-
 Photorhabdus luminescens myristic acid + FMN + H2O + light
-
 ?
1.14.14.3 octanal + FMNH + O2
-
 Photorhabdus luminescens octanoic acid + FMN + H2O + light
-
 ir

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.3 0.000015
-
 FMNH myristic aldehyde as second substrate Photorhabdus luminescens
1.14.14.3 0.000025
-
 FMNH dodecanoic aldehyde as second substrate Photorhabdus luminescens
1.14.14.3 0.0000317
-
 FMNH decanoic aldehyde as second substrate Photorhabdus luminescens