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Literature summary extracted from

  • Saito, Y.; Hayaishi, O.; Rothenberg, S.
    Studies on oxygenases: Enzymatic formation of 3-hydroxy-L-kynurenine from L-kynurenine (1957), J. Biol. Chem., 229, 921-934.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.9 azide 0.005 M Rattus norvegicus
1.14.13.9 thiocyanate 0.01 M Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.9 CN- inhibition at 0.01 M Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.9 0.023
-
 NADPH
-
 Rattus norvegicus
1.14.13.9 0.025
-
 kynurenine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.9 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.9 Br- strong stimulation Rattus norvegicus
1.14.13.9 Cl- strong stimulation at 0.02 M Rattus norvegicus
1.14.13.9 CN- stimulates Rattus norvegicus
1.14.13.9 F- stimulates Rattus norvegicus
1.14.13.9 I- stimulates Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.9 L-kynurenine + NADPH + O2 Rattus norvegicus
-
 ? + NADP+
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2 Rattus norvegicus Osborne-Mendel
-
 ? + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.9 Rattus norvegicus
-
 Osborne-Mendel strain
-
1.14.13.9 Rattus norvegicus Osborne-Mendel
-
 Osborne-Mendel strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.9 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.9 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.9 L-kynurenine + NADPH + O2 no reaction with D-isomer Rattus norvegicus 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2 no reaction with D-isomer Rattus norvegicus Osborne-Mendel 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2
-
 Rattus norvegicus ? + NADP+
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2
-
 Rattus norvegicus Osborne-Mendel ? + NADP+
-
 ?
1.14.13.9 o-hydroxybenzoyl-DL-alanine + NADPH + O2 about 25% of the activity with L-kynurenine Rattus norvegicus ? + NADP+
-
 ?
1.14.13.9 o-hydroxybenzoyl-DL-alanine + NADPH + O2 about 25% of the activity with L-kynurenine Rattus norvegicus Osborne-Mendel ? + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.9 23
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.9 8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.9 7 9.3 about 50% of maximal activity at pH 7 and 9.3 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.9 FAD flavoprotein Rattus norvegicus
1.14.13.9 NADPH
-
 Rattus norvegicus