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Literature summary extracted from

  • Ziegler, D.M.; Poulsen, L.L.
    Hepatic microsomal mixed-function amine oxidase (1978), Methods Enzymol., 52, 142-151.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.8 Guanidines stimulate NADPH- and O2-dependent oxidation of tertiary amines and sulfur-containing substrates with alkyl side-chains of less than 5 carbons Sus scrofa
1.14.13.8 Lipophilic primary alkylamines stimulate NADPH- and O2-dependent oxidation of tertiary amines and sulfur-containing substrates with alkyl side-chain of less than 5 carbons Sus scrofa
1.14.13.8 n-octylamine stimulation of tert-amine oxidation, not sec-amine oxidation Sus scrofa
1.14.13.8 tertiary amines self-activation with alkyl side chains of 7 or more Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.8 anionic detergents
-
 Sus scrofa
1.14.13.8 fatty acids
-
 Sus scrofa
1.14.13.8 SDS
-
 Sus scrofa
1.14.13.8 stearate
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.8 65000
-
 8 * 65000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.8 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.8
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.8 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.8 1.43
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.14.13.8 -15°C, several months with little or no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.8 methimazole + NADPH + O2
-
 Sus scrofa N-methylmethimidazole-2-sulfinic acid + NADP+ + H2O
-
 ?
1.14.13.8 additional information catalyzes NADPH- and O2-dependent N-oxidation of N-substituted amines and hydrazines and the S-oxidation of thioureylenes and thiols Sus scrofa ?
-
 ?
1.14.13.8 N,N-dimethylaniline + NADPH + O2
-
 Sus scrofa N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.8 octamer 8 * 65000, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.8 FAD flavoprotein Sus scrofa
1.14.13.8 NADH
-
 Sus scrofa
1.14.13.8 NADPH
-
 Sus scrofa