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Literature summary extracted from

  • Halkier, B.A.; Moller, B.L.
    The biosynthesis of cyanogenic glucosides in higher plants. Identification of three hydroxylation steps in the biosynthesis of dhurrin in Sorghum bicolor (L.) Moench and the involvement of 1-ACI-nitro-2-(p-hydroxyphenyl)ethane as an intermediate (1990), J. Biol. Chem., 265, 21114-21121.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.36 0.05
-
 1-nitro-2-(4-hydroxyphenyl)ethane pH 7.9, 30°C Sorghum bicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.36 L-tyrosine + O2 + [reduced NADPH-hemoprotein reductase] Sorghum bicolor enzyme in biosynthesis of cyanogenic glucosides N-hydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.36 Sorghum bicolor
-
-
-
1.14.14.36 Sorghum bicolor Q43135
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.36 seedling
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.36 1-nitro-2-(4-hydroxyphenyl)ethane + O2 + [reduced NADPH-hemoprotein reductase] involvement of 1-nitro-2-(4-hydroxyphenyl)ethane or more likely its aci-nitro tautomer as an intermediate between N-hydroxytyrosine and 4-hydroxyphenylacetaldehyde oxime Sorghum bicolor N,N-dihydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.36 L-tyrosine + O2 + [reduced NADPH-hemoprotein reductase]
-
 Sorghum bicolor N-hydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.36 L-tyrosine + O2 + [reduced NADPH-hemoprotein reductase] enzyme in biosynthesis of cyanogenic glucosides Sorghum bicolor N-hydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.36 N,N-dihydroxy-L-tyrosine
-
 Sorghum bicolor (E)-[4-hydroxyphenylacetaldehyde oxime] + CO2 + H2O
-
 ?
1.14.14.36 N-hydroxy-L-tyrosine + O2 + [reduced NADPH-hemoprotein reductase] involvement of 1-nitro-2-(4-hydroxyphenyl)ethane or more likely its aci-nitro tautomer as an intermediate between N-hydroxytyrosine and 4-hydroxyphenylacetaldehyde oxime Sorghum bicolor N,N-dihydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.36 CYP79A1
-
 Sorghum bicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.36 10 40
-
 Sorghum bicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.36 heme a heme-thiolate protein Sorghum bicolor
1.14.14.36 NADPH
-
 Sorghum bicolor