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Literature summary extracted from

  • Ameyama, M.; Shinagawa, E.; Matsushita, K.; Adachi, O.
    D-Fructose dehydrogenase of Gluconobacter industrius: purification, characterization, and application to enzymatic microdetermination of D-fructose (1981), J. Bacteriol., 145, 814-823.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.5.14 analysis the enzyme is a satisfactory reagent for microdetermination of D-fructose Gluconobacter oxydans

General Stability

EC Number General Stability Organism
1.1.5.14 detergents stabilize Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.14 10
-
 D-fructose
-
 Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.14 19700
-
 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans
1.1.5.14 50800
-
 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans
1.1.5.14 67000
-
 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.14 D-fructose + acceptor Gluconobacter oxydans involved in utilization of D-fructose by acetic acid bacteria 5-dehydro-D-fructose + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.14 Gluconobacter oxydans
-
 IFO 3260
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.14 172
-
-
 Gluconobacter oxydans

Storage Stability

EC Number Storage Stability Organism
1.1.5.14 -20°C or below, activity completely preserved Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.14 D-fructose + 2,6-dichlorophenolindophenol
-
 Gluconobacter oxydans 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.14 D-fructose + acceptor involved in utilization of D-fructose by acetic acid bacteria Gluconobacter oxydans 5-dehydro-D-fructose + reduced acceptor
-
 ?
1.1.5.14 D-fructose + ferricyanide
-
 Gluconobacter oxydans 5-dehydro-D-fructose + ferrocyanide
-
 ?
1.1.5.14 D-fructose + nitro blue tetrazolium
-
 Gluconobacter oxydans 5-dehydro-D-fructose + ?
-
 ?
1.1.5.14 D-fructose + phenazine methosulfate
-
 Gluconobacter oxydans 5-dehydro-D-fructose + ?
-
 ?
1.1.5.14 additional information no activity with NADP+ Gluconobacter oxydans ?
-
 ?
1.1.5.14 additional information no activity with O2 Gluconobacter oxydans ?
-
 ?
1.1.5.14 additional information no activity with NAD+ Gluconobacter oxydans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.14 trimer 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.14 25
-
-
 Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.14 35
-
 rapid inactivation beyond Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.14 4 4.5
-
 Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.5.14 4.5 6 stable Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.14 cytochrome c contains a cytochrome c like electron carrier, tightly bound Gluconobacter oxydans