EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.2.3 | - |
Saccharomyces cerevisiae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.2.3 | - |
Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.2.3 | R376K | mutant enzyme shows no activity | Saccharomyces cerevisiae |
1.1.2.3 | Y254F | significantly reduced activity of mutant enzyme | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.3 | 0.084 | - |
Phenyllactate | Y254F mutant | Saccharomyces cerevisiae | |
1.1.2.3 | 0.35 | - |
L-lactate | Y254F mutant | Saccharomyces cerevisiae | |
1.1.2.3 | 0.4 | - |
Phenyllactate | wild type enzyme | Saccharomyces cerevisiae | |
1.1.2.3 | 0.49 | - |
L-lactate | wild type enzyme | Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.3 | Fe2+ | bound to enzyme | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.3 | (S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.3 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.3 | (S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
1.1.2.3 | (S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
1.1.2.3 | additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? | |
1.1.2.3 | phenyllactate + ferricytochrome c | - |
Saccharomyces cerevisiae | phenylpyruvate + ferrocytochrome c | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.3 | 0.29 | - |
Phenyllactate | Y254F mutant enzyme | Saccharomyces cerevisiae | |
1.1.2.3 | 6.1 | - |
L-lactate | Y254F mutant enzyme | Saccharomyces cerevisiae | |
1.1.2.3 | 16.5 | - |
Phenyllactate | wild type enzyme | Saccharomyces cerevisiae | |
1.1.2.3 | 270 | - |
L-lactate | wild type enzyme | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.3 | FMN | - |
Saccharomyces cerevisiae | |
1.1.2.3 | heme | - |
Saccharomyces cerevisiae | |
1.1.2.3 | additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae |