BRENDA - Enzyme Database

Crystal structures of two tropinone reductases: Different reaction stereospecificities in the same protein fold

Nakajima, K.; Yamashita, A.; Akama, H.; Nakatsu, T.; Kato, H.; Hashimoto, T.; Oda, J.; Yamada, Y.; Proc. Natl. Acad. Sci. USA 95, 4876-4881 (1998) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.1.206
-
Datura stramonium
1.1.1.236
TRII cDNA subcloned from pTR2EN induced in Escherichia coli BL21(DE3)
Datura stramonium
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
1.1.1.206
hanging-drop vapor diffusion at 20°C
Datura stramonium
1.1.1.236
TrII is crystallized by hanging-drop vapor diffusion with 2-methyl-2,4-pentanediol as precipitating reagent, crystals grown by macroseeding technique, crystals belong to the P4.2.2.1.2 space group with cell dimensions of a = b = 62.8 A and c = 128.4 A
Datura stramonium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.236
tropinone + NADPH + H+
Datura stramonium
-
pseudotropine + NADP+
-
-
?
1.1.1.236
tropinone + NADPH + H+
Hyoscyamus niger
-
pseudotropine + NADP+
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.206
Datura stramonium
-
-
-
1.1.1.236
Datura stramonium
-
-
-
1.1.1.236
Hyoscyamus niger
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.206
-
Datura stramonium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.206
tropine + NADP+
-
348028
Datura stramonium
tropinone + NADPH
-
348028
Datura stramonium
?
1.1.1.236
tropinone + NADPH + H+
-
348028
Datura stramonium
pseudotropine + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
348028
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.206
dimer
two very similar subunits, crystallization
Datura stramonium
1.1.1.236
dimer
-
Datura stramonium
1.1.1.236
dimer
-
Hyoscyamus niger
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.206
-
Datura stramonium
1.1.1.236
TRII cDNA subcloned from pTR2EN induced in Escherichia coli BL21(DE3)
Datura stramonium
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.206
hanging-drop vapor diffusion at 20°C
Datura stramonium
1.1.1.236
TrII is crystallized by hanging-drop vapor diffusion with 2-methyl-2,4-pentanediol as precipitating reagent, crystals grown by macroseeding technique, crystals belong to the P4.2.2.1.2 space group with cell dimensions of a = b = 62.8 A and c = 128.4 A
Datura stramonium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.236
tropinone + NADPH + H+
Datura stramonium
-
pseudotropine + NADP+
-
-
?
1.1.1.236
tropinone + NADPH + H+
Hyoscyamus niger
-
pseudotropine + NADP+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.206
-
Datura stramonium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.206
tropine + NADP+
-
348028
Datura stramonium
tropinone + NADPH
-
348028
Datura stramonium
?
1.1.1.236
tropinone + NADPH + H+
-
348028
Datura stramonium
pseudotropine + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
348028
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.206
dimer
two very similar subunits, crystallization
Datura stramonium
1.1.1.236
dimer
-
Datura stramonium
1.1.1.236
dimer
-
Hyoscyamus niger