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Literature summary extracted from

  • Portsteffen, A.; Draeger, B.; Nahrstedt, A.
    The reduction of tropinone in Datura stramonium root cultures by two specific reductases (1994), Phytochemistry, 37, 391-400.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.236
-
 Datura stramonium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.236 0.016
-
 NADPH
-
 Datura stramonium
1.1.1.236 0.06
-
 tropinone pH 7.5 Datura stramonium
1.1.1.236 0.063
-
 tropinone pH 7.9 Datura stramonium
1.1.1.236 0.07
-
 tropinone pH 6.7 Datura stramonium
1.1.1.236 0.1
-
 tropinone pH 7.0 Datura stramonium
1.1.1.236 0.11
-
 tropinone pH 6.4 Datura stramonium
1.1.1.236 0.33
-
 tropinone pH 5.95 Datura stramonium
1.1.1.236 1.25
-
 tropinone pH 5.4 Datura stramonium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.236 27500
-
 3 * 27500 Atropa belladonna
1.1.1.236 27700
-
 3 * 27700, SDS-PAGE Datura stramonium
1.1.1.236 29000
-
 3 * 29000 Datura stramonium
1.1.1.236 77700
-
 gel filtration Datura stramonium
1.1.1.236 78500
-
-
 Atropa belladonna

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.236 tropinone + NADPH + H+ Atropa belladonna
-
 pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ Datura stramonium pseudotropine oxidation never observed pseudotropine + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.236 Atropa belladonna
-
-
-
1.1.1.236 Datura stramonium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.236
-
 Datura stramonium
1.1.1.236
-
 Atropa belladonna

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.236 root
-
 Atropa belladonna
-
1.1.1.236 root hairy roots Datura stramonium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.236 8.16
-
-
 Datura stramonium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.236 4-ethyl-cyclohexanone + NADPH
-
 Datura stramonium 4-ethylcyclohexanol + NADP+
-
 ?
1.1.1.236 4-methyl-cyclohexanone + NADPH
-
 Datura stramonium 4-methylcyclohexanol + NADP+
-
 ?
1.1.1.236 6-hydroxytropinone + NADPH
-
 Datura stramonium ? + NADP+
-
 ?
1.1.1.236 N-methyl-4-piperidone + NADPH
-
 Datura stramonium N-methylpiperidin-4-ol + NADP+
-
 ?
1.1.1.236 N-propyl-4-piperidone + NADPH
-
 Datura stramonium N-propylpiperidin-4-ol + NADP+
-
 ?
1.1.1.236 tetrahydrothiopyran-4-one + NADPH
-
 Datura stramonium tetrahydrothiopyran-4-ol + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Atropa belladonna pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ pseudotropine oxidation never observed Datura stramonium pseudotropine + NADP+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.236 trimer 3 * 27500 Atropa belladonna
1.1.1.236 trimer 3 * 29000 Datura stramonium
1.1.1.236 trimer 3 * 27700, SDS-PAGE Datura stramonium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.236 4.5 5 optimum for tetrahydrothiopyran-4-one reduction Datura stramonium
1.1.1.236 6.25
-
-
 Datura stramonium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.236 4 8 keeps 90% of maximal activity in the range pH 5.2-8.2 Datura stramonium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.236 NADPH
-
 Datura stramonium