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Literature summary extracted from

  • Robert, S.; Simier, P.; Fer, A.
    Purification and characterization of mannose 6-phosphate reductase, a potential target for the control of Striga hermonthica and Orobanche ramosa (1999), Aust. J. Plant Physiol., 26, 233-237.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.224 0.0102
-
 NADPH enzyme purified from leaves Striga hermonthica
1.1.1.224 0.0698
-
 NADPH enzyme purified from flowering spikes Phelipanche ramosa
1.1.1.224 5.9
-
 D-mannose 6-phosphate enzyme purified from leaves Striga hermonthica
1.1.1.224 8.6
-
 D-mannose 6-phosphate enzyme purified from flowering spikes Phelipanche ramosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.224 27000
-
 gel filtration with Sephacryl S-200 Phelipanche ramosa
1.1.1.224 33000
-
 gel filtration with Sephacryl S-200 Striga hermonthica

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.224 Phelipanche ramosa
-
 broomrape
-
1.1.1.224 Striga hermonthica
-
 witchweed
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.224
-
 Striga hermonthica
1.1.1.224
-
 Phelipanche ramosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.224 leaf
-
 Striga hermonthica
-
1.1.1.224 spike flowering Phelipanche ramosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.224 0.008
-
 crude extract from mature leaves Striga hermonthica
1.1.1.224 0.014
-
 crude extract from flowering spikes Phelipanche ramosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.224 D-mannose 6-phosphate + NADPH
-
 Striga hermonthica D-mannitol 1-phosphate + NADP+
-
 ?
1.1.1.224 D-mannose 6-phosphate + NADPH
-
 Phelipanche ramosa D-mannitol 1-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.224 monomer 1* 39000, SDS-PAGE Striga hermonthica
1.1.1.224 monomer 1* 39000, SDS-PAGE Phelipanche ramosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.224 35
-
-
 Striga hermonthica
1.1.1.224 45
-
-
 Phelipanche ramosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.224 25 45 75% of maximal activity Striga hermonthica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.224 7
-
-
 Striga hermonthica
1.1.1.224 7
-
-
 Phelipanche ramosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.224 additional information
-
 rapid decrease in the range of basic pH values Striga hermonthica
1.1.1.224 additional information
-
 rapid decrease in the range of basic pH values Phelipanche ramosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.224 NADH 5% activity compared to NADPH Phelipanche ramosa
1.1.1.224 NADH 10% of activity compared to NADPH Striga hermonthica
1.1.1.224 NADPH
-
 Striga hermonthica
1.1.1.224 NADPH
-
 Phelipanche ramosa