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Literature summary extracted from

  • Everard, J.D.; Cantini, C.; Grumet, R.; Plummer, J.; Loescher, W.H.
    Molecular cloning of mannose-6-phosphate reductase and its developmental expression in celery (1997), Plant Physiol., 113, 1427-1435.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.224
-
 Apium graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.224 0.0021
-
 NADPH enzyme purified from leaves Apium graveolens
1.1.1.224 0.0062
-
 NADPH recombinant enzyme Apium graveolens
1.1.1.224 10.1
-
 D-mannose 6-phosphate recombinant enzyme Apium graveolens
1.1.1.224 13.6
-
 D-mannose 6-phosphate enzyme purified from leaves Apium graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.224 35000
-
 2 * 35000, calculated from cDNA sequence Apium graveolens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.224 Apium graveolens
-
 celery
-
1.1.1.224 Apium graveolens
-
 cv. Giant Pascal
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.224 purification of recombinant enzyme Apium graveolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.224 cotyledon 21-d old, low activity Apium graveolens
-
1.1.1.224 flower low activity Apium graveolens
-
1.1.1.224 leaf
-
 Apium graveolens
-
1.1.1.224 additional information no activity in sink tissue, i.e. young root, young petiole, young leaf and mature petiole Apium graveolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.224 additional information
-
 measurement of activity during leaf development Apium graveolens
1.1.1.224 3.93
-
 purified recombinant enzyme Apium graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.224 D-mannose 6-phosphate + NADPH
-
 Apium graveolens D-mannitol 1-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.224 dimer 2 * 35000, calculated from cDNA sequence Apium graveolens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.224 NADPH
-
 Apium graveolens