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Literature summary extracted from

  • Loescher, W.H.; Tyson, R.H.; Everard, J.D.; Redgewell, J.; Bieleski, R.L.
    Mannitol synthesis in higher plants. Evidence for the role and characterization of a NADPH-dependent mannose 6-phosphate reductase (1992), Plant Physiol., 98, 1396-1402.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.224 degassed buffers stabilize Apium graveolens
1.1.1.224 DTT stabilizes Apium graveolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.224 NaCl 47% reduction by 100 mM Apium graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.224 15.8
-
 D-mannose 6-phosphate
-
 Apium graveolens
1.1.1.224 45
-
 D-mannitol 1-phosphate
-
 Apium graveolens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.224 cytoplasm
-
 Apium graveolens 5737
-
1.1.1.224 cytoplasm
-
 Ligustrum vulgare 5737
-
1.1.1.224 protoplast
-
 Apium graveolens
-
-
1.1.1.224 protoplast
-
 Ligustrum vulgare
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.224 35000
-
 2 * 35000, SDS-PAGE Apium graveolens
1.1.1.224 53000
-
 gel filtration with Superose 12 Apium graveolens
1.1.1.224 58000
-
 gel filtration with Sephacryl S-200 Apium graveolens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.224 Apium graveolens
-
 celery
-
1.1.1.224 Apium graveolens
-
 cv. Giant Pascal
-
1.1.1.224 Ligustrum vulgare
-
 privet
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.224 DTT stabilizes Apium graveolens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.224
-
 Apium graveolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.224 leaf
-
 Apium graveolens
-
1.1.1.224 shoot
-
 Ligustrum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.224 0.053
-
-
 Apium graveolens
1.1.1.224 3.76
-
 purified from leaves Apium graveolens

Storage Stability

EC Number Storage Stability Organism
1.1.1.224 -21°C, 50% activity retained after 1 month Apium graveolens
1.1.1.224 4°C, lyophilized active fractions stable for several months Apium graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.224 D-mannose 6-phosphate + NADPH
-
 Apium graveolens D-mannitol 1-phosphate + NADP+
-
 r
1.1.1.224 D-mannose 6-phosphate + NADPH no activity with D-fructose 6-phosphate, D-glucose 6-phosphate, D-fructose 1-phosphate, D-mannose 1-phosphate, D-mannose Ligustrum vulgare D-mannitol 1-phosphate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.224 dimer 2 * 35000, SDS-PAGE Apium graveolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.224 7.5
-
 D-mannose 6-phosphate Apium graveolens
1.1.1.224 8.5
-
 D-mannitol 1-phosphate Apium graveolens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.224 6 9 inactivated at pH below 6 Apium graveolens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.224 6 9
-
 Apium graveolens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.224 NADP+ NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate Apium graveolens
1.1.1.224 NADP+ NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate Ligustrum vulgare
1.1.1.224 NADPH NADH cannot replace NADPH in reducing mannose 6-phosphate Apium graveolens
1.1.1.224 NADPH NADH cannot replace NADPH in reducing mannose 6-phosphate Ligustrum vulgare