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Literature summary extracted from

  • Heller, W.; Forkmann, G.; Britsch, L.; Grisebach, H.
    Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis (1985), Planta, 165, 284-287.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.219 additional information Matthiola incana reaction in anthocyanidin biosynthesis in plants ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Matthiola incana
-
 gillyflower
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 flower
-
 Matthiola incana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.219 0.018
-
-
 Matthiola incana

Storage Stability

EC Number Storage Stability Organism
1.1.1.219 -70°C, 10% glycerol, crude extract stable for several weeks Matthiola incana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (+)-dihydrokaempferol + NADPH
-
 Matthiola incana cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (+)-dihydromyricetin + NADPH i.e. 5'-hydroxy-dihydroquercetin Matthiola incana cis-3,4-leucodelphinidin + NADP+ i.e. 5,7,4'-trihydroxyflavan-3,4-cis-diol ?
1.1.1.219 (+)-dihydroquercetin + NADPH
-
 Matthiola incana cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 additional information reaction in anthocyanidin biosynthesis in plants Matthiola incana ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.219 25
-
 assay at Matthiola incana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.219 5.5 6.5 broad optimum Matthiola incana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.219 5.1 7.2 about half-maximal activity at pH 5.1 and 7.2 Matthiola incana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 additional information FMN, FAD or 6,7-dimethyl-5,6,7,8-tetrahydropterine cannot replace NADPH Matthiola incana
1.1.1.219 NADH half as effective as NADPH Matthiola incana
1.1.1.219 NADPH
-
 Matthiola incana