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Literature summary extracted from

  • Mallonee, D.H.; Lijewski, M.A.; Hylemon, P.B.
    Expression in Escherichia coli and characterization of a bile acid-inducible 3alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708 (1995), Curr. Microbiol., 30, 259-263.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.213 additional information bile acid-incucible baiA gene family Eubacterium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.50 overexpression in Escherichia coli Eubacterium sp.
1.1.1.213 overexpressed in Escherichia coli HMS174(DE3) Eubacterium sp.
1.1.1.395 expressed in Escherichia coli Hms174(DE3) cells Eubacterium sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.213 additional information potential CoA binding sites spanning residues 182 to 193. N-terminal region is thought to contain the NAD(P)+ binding site, the middle and C-terminal regions the active site of the enzyme Eubacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 27000
-
-
 Eubacterium sp.
1.1.1.213 27000
-
 PAGE, western blot Eubacterium sp.
1.1.1.395 27000
-
 x * 27000, SDS-PAGE Eubacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Eubacterium sp.
-
-
-
1.1.1.50 Eubacterium sp. VPI 12708
-
-
-
1.1.1.213 Eubacterium sp.
-
-
-
1.1.1.213 Eubacterium sp. VPI 12708
-
-
-
1.1.1.395 Eubacterium sp.
-
-
-
1.1.1.395 Eubacterium sp. VPI 12708
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50 partial Eubacterium sp.
1.1.1.213 Centriprep-10 concentration, DEAE-HPLC Eubacterium sp.
1.1.1.395 AP-2 DEAE column chromatography Eubacterium sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.213 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ short-chain alcohol/polyol dehydrogenase family Eubacterium sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 cholic acid + NAD(P)+
-
 Eubacterium sp. 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.50 cholic acid + NAD(P)+
-
 Eubacterium sp. VPI 12708 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.213 cholic acid + NAD(P)+
-
 Eubacterium sp. 3-oxocholate + NAD(P)H very little activity ?
1.1.1.213 cholic acid + NAD(P)+
-
 Eubacterium sp. VPI 12708 3-oxocholate + NAD(P)H very little activity ?
1.1.1.213 choloyl-CoA + NAD(P)+
-
 Eubacterium sp. 3-oxocholoyl-CoA + NAD(P)H + H+
-
 r
1.1.1.213 choloyl-CoA + NAD(P)+
-
 Eubacterium sp. VPI 12708 3-oxocholoyl-CoA + NAD(P)H + H+
-
 r
1.1.1.213 deoxycholoyl-CoA + NAD(P)+ 75% activity compared with cholyl-CoA Eubacterium sp. 3-oxodeoxycholoyl-CoA + NAD(P)H + H+
-
 ?
1.1.1.213 deoxycholoyl-CoA + NAD(P)+ 75% activity compared with cholyl-CoA Eubacterium sp. VPI 12708 3-oxodeoxycholoyl-CoA + NAD(P)H + H+
-
 ?
1.1.1.213 additional information no activity with cholate, chenodeoxycholate, deoxycholate, glycocholate, taurocholate, ursodeoxycholate and bile acid Eubacterium sp. ?
-
 ?
1.1.1.213 additional information no activity with cholate, chenodeoxycholate, deoxycholate, glycocholate, taurocholate, ursodeoxycholate and bile acid Eubacterium sp. VPI 12708 ?
-
 ?
1.1.1.395 cholic acid + NAD+
-
 Eubacterium sp. ? + NADH + H+
-
 ?
1.1.1.395 choloyl-CoA + NAD+ preferred substrate Eubacterium sp. 3-oxocholoyl-CoA + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 27000, SDS-PAGE Eubacterium sp.
1.1.1.213 tetramer
-
 Eubacterium sp.
1.1.1.395 ? x * 27000, SDS-PAGE Eubacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.395 baiA1
-
 Eubacterium sp.
1.1.1.395 bile acid-inducible 3 alpha-hydroxysteroid dehydrogenase
-
 Eubacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD+
-
 Eubacterium sp.
1.1.1.50 NADP+
-
 Eubacterium sp.
1.1.1.395 NAD+ preferred cofactor Eubacterium sp.