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Literature summary extracted from

  • Tomkins, G.M.
    A mammalian 3alpha-hydroxysteroid dehydrogenase (1956), J. Biol. Chem., 218, 437-447.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.213 Cu2+ 100% inhibition at 0.1 mM Rattus norvegicus
1.1.1.213 iodoacetate 50% inhibition at 0.1 mM Rattus norvegicus
1.1.1.213 p-chloromercuribenzoate non-competitive with respect to dihydrocortisone 46-100% inhibition at 0.01 mM, preincubation with NADH lowers the inhibitory effect Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.213 0.01
-
 5beta-pregnane-17alpha,20beta,21-triol-3,11-dione
-
 Rattus norvegicus
1.1.1.213 0.015
-
 NADH
-
 Rattus norvegicus
1.1.1.213 0.04
-
 NAD+
-
 Rattus norvegicus
1.1.1.213 0.04
-
 NADP+
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.213 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.213 ammonium sulfate precipitation, Ca2(PO4)2 gel extraction, ethanol precipitation Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.213 liver also present in kidney and testis Rattus norvegicus
-
1.1.1.213 additional information low activities found in dog and rabbit liver, no significant activities are detected in extracts of beef, hog, guinea pig, mouse and human liver Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.213 -10°C, 3 weeks, activity declines about 50% Rattus norvegicus
1.1.1.213 -10°C, most purified preparation, 5 days, loses between 80% and 100% of activity Rattus norvegicus
1.1.1.213 3°C, first ammonium sulfate fraction, stable at least 3 weeks Rattus norvegicus
1.1.1.213 3°C, most purified preparation, 5 days, loses 50% of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.213 5beta-pregnane-17alpha,20beta,21-triol-3,11-dione + NAD(P)H
-
 Rattus norvegicus 5beta-pregnane-3alpha,17alpha,20beta,21-tetraol-11-one + NAD(P)+
-
 r
1.1.1.213 androstan-17beta-ol-3-one + NADH
-
 Rattus norvegicus androstan-3alpha,17beta-diol + NAD+
-
 ?
1.1.1.213 androstan-3,17-dione + NADH
-
 Rattus norvegicus androstan-3alpha-ol-17-one + NAD+
-
 ?
1.1.1.213 etiocholane-3,17-dione + NADH
-
 Rattus norvegicus etiocholan-3alpha-ol-17-one + NAD+
-
 ?
1.1.1.213 pregnane-11beta,17alpha,21-triol-3,20-dione + NADH
-
 Rattus norvegicus pregnane-3alpha,11beta,17alpha,21-tetrol-20-one + NAD+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.213 NAD+
-
 Rattus norvegicus
1.1.1.213 NADH
-
 Rattus norvegicus
1.1.1.213 NADP+
-
 Rattus norvegicus
1.1.1.213 NADPH
-
 Rattus norvegicus