BRENDA - Enzyme Database

Specificities of the enzymes of N-alkyltropane biosynthesis in Brugmansia and Datura

Boswell, H.D.; Dräger, B.; McLauchlan W.R.; Portsteffen, A.; Robins D.J.; Robins R.J.; Walton, N.J.; Phytochemistry 52, 871-878 (1999) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.236
0.11
-
tropinone
-
Datura stramonium
1.1.1.236
0.16
-
tropinone
-
Brugmansia x candida
1.1.1.236
0.25
-
N-ethylnortropinone
-
Datura stramonium
1.1.1.236
0.67
-
N-(2-fluoroethyl)nortropinone
-
Datura stramonium
1.1.1.236
1.4
-
N-methylpiperid-4-one
-
Datura stramonium
1.1.1.236
2.8
-
4-methylcyclohexanone
-
Datura stramonium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.236
tropinone + NADPH + H+
Datura stramonium
-
pseudotropine + NADP+
-
-
?
1.1.1.236
tropinone + NADPH + H+
Hyoscyamus niger
-
pseudotropine + NADP+
-
-
?
1.1.1.236
tropinone + NADPH + H+
Brugmansia x candida
-
pseudotropine + NADP+
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.206
Brugmansia sp.
-
candida x aurea hybrid
-
1.1.1.206
Datura stramonium
-
-
-
1.1.1.236
Brugmansia x candida
-
x aurea hybrid
-
1.1.1.236
Datura stramonium
-
D15/5
-
1.1.1.236
Datura stramonium cv. D15/5
-
D15/5
-
1.1.1.236
Hyoscyamus niger
-
-
-
1.4.3.22
Brugmansia x candida
-
hybrid
-
2.3.1.185
Brugmansia x candida
-
Brugmansia aurea x Brugmansia versicolor
-
2.3.1.186
Brugmansia x candida
-
Brugmansia aurea x Brugmansia versicolor
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.206
-
Datura stramonium
1.1.1.206
-
Brugmansia sp.
1.1.1.236
-
Datura stramonium
1.1.1.236
-
Brugmansia x candida
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.206
root
-
Datura stramonium
-
1.1.1.206
root
-
Brugmansia sp.
-
1.1.1.236
root
-
Datura stramonium
-
1.1.1.236
root
-
Hyoscyamus niger
-
1.1.1.236
root
-
Brugmansia x candida
-
1.4.3.22
hairy root culture
-
Brugmansia x candida
-
2.3.1.185
root
root culture
Brugmansia x candida
-
2.3.1.186
root
root culture culture
Brugmansia x candida
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.185
0.00075
-
activity with tropine and acetyl-CoA
Brugmansia x candida
2.3.1.185
0.001
-
activity with tropine and tigloyl-CoA
Brugmansia x candida
2.3.1.186
0.0035
-
activity with pseudotropine and acetyl-CoA
Brugmansia x candida
2.3.1.186
0.025
-
activity with pseudotropine and tigloyl-CoA
Brugmansia x candida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.206
3-methylcyclohexanone + NADPH
-
347973
Datura stramonium
3-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
3-methylcyclohexanone + NADPH
-
347973
Brugmansia sp.
3-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
3-quinuclidinone + NADPH + H+
-
347973
Datura stramonium
quinuclidin-3-ol + NADP+
-
-
-
?
1.1.1.206
3-quinuclidinone + NADPH + H+
-
347973
Brugmansia sp.
quinuclidin-3-ol + NADP+
-
-
-
?
1.1.1.206
4-ethylcyclohexanone + NADPH
-
347973
Datura stramonium
4-ethylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-ethylcyclohexanone + NADPH
-
347973
Brugmansia sp.
4-ethylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-ethylcyclohexanone + NADPH
-
347973
Datura stramonium cv. D15/5
4-ethylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-methylcyclohexanone + NADPH
-
347973
Datura stramonium
4-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-methylcyclohexanone + NADPH
-
347973
Brugmansia sp.
4-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-methylcyclohexanone + NADPH
-
347973
Datura stramonium cv. D15/5
4-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
7-hydroxytropinone + NADPH
-
347973
Datura stramonium
7-hydroxytropan-3-ol + NADP+
-
-
-
?
1.1.1.206
7-hydroxytropinone + NADPH
-
347973
Brugmansia sp.
7-hydroxytropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-(2-fluoroethyl)nortropinone + NADPH
-
347973
Datura stramonium
N-(2-fluoroethyl)nortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-(2-fluoroethyl)nortropinone + NADPH
-
347973
Brugmansia sp.
N-(2-fluoroethyl)nortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-isopropylnortropinone + NADPH
-
347973
Datura stramonium
N-isopropylnortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-isopropylnortropinone + NADPH
-
347973
Brugmansia sp.
N-isopropylnortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-methyl-4-piperidone + NADPH
-
347973
Datura stramonium
1-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.206
N-methyl-4-piperidone + NADPH
-
347973
Brugmansia sp.
1-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.206
N-methyl-4-piperidone + NADPH
-
347973
Datura stramonium cv. D15/5
1-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.206
nortropine + NADP+
-
347973
Datura stramonium
nortropinone + NADPH
-
347973
Datura stramonium
?
1.1.1.206
nortropine + NADP+
-
347973
Brugmansia sp.
nortropinone + NADPH
-
347973
Brugmansia sp.
r
1.1.1.206
nortropine + NADP+
-
347973
Datura stramonium cv. D15/5
nortropinone + NADPH
-
347973
Datura stramonium cv. D15/5
?
1.1.1.206
tropine + NADP+
-
347973
Datura stramonium
tropinone + NADPH
-
347973
Datura stramonium
r
1.1.1.206
tropine + NADP+
-
347973
Brugmansia sp.
tropinone + NADPH
-
347973
Brugmansia sp.
r
1.1.1.206
tropine + NADP+
-
347973
Datura stramonium cv. D15/5
tropinone + NADPH
-
347973
Datura stramonium cv. D15/5
r
1.1.1.236
N-(2-fluoroethyl)nortropinone + NADPH
-
347973
Datura stramonium
? + NADP+
-
-
-
?
1.1.1.236
N-ethylnortropinone + NADPH
-
347973
Datura stramonium
? + NADP+
-
-
-
?
1.1.1.236
N-iso-propylnortropinone + NADPH
-
347973
Datura stramonium
? + NADP+
-
-
-
?
1.1.1.236
N-methyl-4-piperidone + NADPH
-
347973
Datura stramonium
N-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.236
N-methyl-4-piperidone + NADPH
-
347973
Hyoscyamus niger
N-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
347973
Datura stramonium
pseudotropine + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
347973
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
347973
Brugmansia x candida
pseudotropine + NADP+
-
-
-
?
1.4.3.22
1,4-diaminobutane + H2O + O2
56% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
4-aminobutanal + NH3 + H2O2
-
-
-
?
1.4.3.22
cadaverine + H2O + O2
15% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
5-aminopentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-(2-fluoroethyl)putrescine + H2O + O2
same activity as with N-methylputrescine as substrate
347973
Brugmansia x candida
N-(2-fluoroethyl)aminobutanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-ethylcadaverine + H2O + O2
17% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
5-(ethylamino)pentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-ethylputrescine + H2O + O2
same activity as with N-methylputrescine as substrate
347973
Brugmansia x candida
4-(ethylamino)butanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-methylcadaverine + H2O + O2
16% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
5-(methylamino)pentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-methylputrescine + H2O + O2
best substrate
347973
Brugmansia x candida
4-(methylamino)butanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-n-propylputrescine + H2O + O2
50% of the activity compared to N-methylputrescine as substrate
347973
Brugmansia x candida
4-propylaminobutanal + NH3 + H2O2
-
-
-
?
2.3.1.185
acetyl-CoA + N-ethylnortropan-3-ol
16% of the activity with acetyl-CoA and tropine
347973
Brugmansia x candida
CoA + 8-ethyl-8-azabicyclo[3.2.1]oct-3-yl-acetate
-
-
-
?
2.3.1.185
acetyl-CoA + tropine
-
347973
Brugmansia x candida
CoA + O-acetyltropine
-
-
-
?
2.3.1.185
additional information
no activity with pseudotropine
347973
Brugmansia x candida
?
-
-
-
?
2.3.1.185
tigloyl-CoA + N-ethylnortropan-3-ol
76% of the activity with tigloyl-CoA and tropine
347973
Brugmansia x candida
CoA + 8-ethyl-8-azabicyclo[3.2.1]oct-3-yl (2E)-2-methylbut-2-enoate
-
-
-
?
2.3.1.185
tigloyl-CoA + norpseudopelletierin-3-ol
5% of the activity with tigloyl-CoA and tropine
347973
Brugmansia x candida
CoA + ?
-
-
-
?
2.3.1.185
tigloyl-CoA + tropine
-
347973
Brugmansia x candida
CoA + O-tigloyltropine
-
-
-
?
2.3.1.186
acetyl-CoA + N-(2-fluoroethyl)nortropan-3-ol
16% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-(2-fluoroethyl)nortropan-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + N-ethylnorpseudopelletierin-3-ol
% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-ethylnorpseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + N-ethylnortropan-3-ol
35% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-ethylnortropan-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + N-iso-propylnorpseudopelletierin-3-ol
7% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-iso-propylnorpseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + norpseudopelletierin-3-ol
9% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + norpseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + pseudopelletierin-3-ol
% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + pseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + pseudotropine
-
347973
Brugmansia x candida
CoA + O-acetylpseudotropine
-
-
-
?
2.3.1.186
additional information
no activity with tropine
347973
Brugmansia x candida
?
-
-
-
?
2.3.1.186
tigloyl-CoA + N-(2-fluoroethyl)nortropan-3-ol
74% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-(2-fluoroethyl)nortropan-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + N-ethylnorpseudopelletierin-3-ol
% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-ethylnorpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + N-ethylnortropan-3-ol
81% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-ethylnortropan-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + N-iso-propylnorpseudopelletierin-3-ol
2% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-iso-propylnorpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + norpseudopelletierin-3-ol
13% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-norpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + norpseudopelletierin-3-ol
40% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-norpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + pseudopelletierin-3-ol
% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-pseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + pseudotropine
-
347973
Brugmansia x candida
CoA + O-tigloylpseudotropine
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.4.3.22
MPO
-
Brugmansia x candida
1.4.3.22
N-methylputrescine oxidase
-
Brugmansia x candida
2.3.1.185
tropine:acyl-CoA transferase
-
Brugmansia x candida
2.3.1.186
pseudotropine:acyl-CoA transferase
-
Brugmansia x candida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.236
0.11
-
tropinone
-
Datura stramonium
1.1.1.236
0.16
-
tropinone
-
Brugmansia x candida
1.1.1.236
0.25
-
N-ethylnortropinone
-
Datura stramonium
1.1.1.236
0.67
-
N-(2-fluoroethyl)nortropinone
-
Datura stramonium
1.1.1.236
1.4
-
N-methylpiperid-4-one
-
Datura stramonium
1.1.1.236
2.8
-
4-methylcyclohexanone
-
Datura stramonium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.236
tropinone + NADPH + H+
Datura stramonium
-
pseudotropine + NADP+
-
-
?
1.1.1.236
tropinone + NADPH + H+
Hyoscyamus niger
-
pseudotropine + NADP+
-
-
?
1.1.1.236
tropinone + NADPH + H+
Brugmansia x candida
-
pseudotropine + NADP+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.206
-
Datura stramonium
1.1.1.206
-
Brugmansia sp.
1.1.1.236
-
Datura stramonium
1.1.1.236
-
Brugmansia x candida
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.206
root
-
Datura stramonium
-
1.1.1.206
root
-
Brugmansia sp.
-
1.1.1.236
root
-
Datura stramonium
-
1.1.1.236
root
-
Hyoscyamus niger
-
1.1.1.236
root
-
Brugmansia x candida
-
1.4.3.22
hairy root culture
-
Brugmansia x candida
-
2.3.1.185
root
root culture
Brugmansia x candida
-
2.3.1.186
root
root culture culture
Brugmansia x candida
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.185
0.00075
-
activity with tropine and acetyl-CoA
Brugmansia x candida
2.3.1.185
0.001
-
activity with tropine and tigloyl-CoA
Brugmansia x candida
2.3.1.186
0.0035
-
activity with pseudotropine and acetyl-CoA
Brugmansia x candida
2.3.1.186
0.025
-
activity with pseudotropine and tigloyl-CoA
Brugmansia x candida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.206
3-methylcyclohexanone + NADPH
-
347973
Datura stramonium
3-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
3-methylcyclohexanone + NADPH
-
347973
Brugmansia sp.
3-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
3-quinuclidinone + NADPH + H+
-
347973
Datura stramonium
quinuclidin-3-ol + NADP+
-
-
-
?
1.1.1.206
3-quinuclidinone + NADPH + H+
-
347973
Brugmansia sp.
quinuclidin-3-ol + NADP+
-
-
-
?
1.1.1.206
4-ethylcyclohexanone + NADPH
-
347973
Datura stramonium
4-ethylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-ethylcyclohexanone + NADPH
-
347973
Brugmansia sp.
4-ethylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-ethylcyclohexanone + NADPH
-
347973
Datura stramonium cv. D15/5
4-ethylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-methylcyclohexanone + NADPH
-
347973
Datura stramonium
4-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-methylcyclohexanone + NADPH
-
347973
Brugmansia sp.
4-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
4-methylcyclohexanone + NADPH
-
347973
Datura stramonium cv. D15/5
4-methylcyclohexanol + NADP+
-
-
-
?
1.1.1.206
7-hydroxytropinone + NADPH
-
347973
Datura stramonium
7-hydroxytropan-3-ol + NADP+
-
-
-
?
1.1.1.206
7-hydroxytropinone + NADPH
-
347973
Brugmansia sp.
7-hydroxytropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-(2-fluoroethyl)nortropinone + NADPH
-
347973
Datura stramonium
N-(2-fluoroethyl)nortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-(2-fluoroethyl)nortropinone + NADPH
-
347973
Brugmansia sp.
N-(2-fluoroethyl)nortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-isopropylnortropinone + NADPH
-
347973
Datura stramonium
N-isopropylnortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-isopropylnortropinone + NADPH
-
347973
Brugmansia sp.
N-isopropylnortropan-3-ol + NADP+
-
-
-
?
1.1.1.206
N-methyl-4-piperidone + NADPH
-
347973
Datura stramonium
1-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.206
N-methyl-4-piperidone + NADPH
-
347973
Brugmansia sp.
1-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.206
N-methyl-4-piperidone + NADPH
-
347973
Datura stramonium cv. D15/5
1-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.206
nortropine + NADP+
-
347973
Datura stramonium
nortropinone + NADPH
-
347973
Datura stramonium
?
1.1.1.206
nortropine + NADP+
-
347973
Brugmansia sp.
nortropinone + NADPH
-
347973
Brugmansia sp.
r
1.1.1.206
nortropine + NADP+
-
347973
Datura stramonium cv. D15/5
nortropinone + NADPH
-
347973
Datura stramonium cv. D15/5
?
1.1.1.206
tropine + NADP+
-
347973
Datura stramonium
tropinone + NADPH
-
347973
Datura stramonium
r
1.1.1.206
tropine + NADP+
-
347973
Brugmansia sp.
tropinone + NADPH
-
347973
Brugmansia sp.
r
1.1.1.206
tropine + NADP+
-
347973
Datura stramonium cv. D15/5
tropinone + NADPH
-
347973
Datura stramonium cv. D15/5
r
1.1.1.236
N-(2-fluoroethyl)nortropinone + NADPH
-
347973
Datura stramonium
? + NADP+
-
-
-
?
1.1.1.236
N-ethylnortropinone + NADPH
-
347973
Datura stramonium
? + NADP+
-
-
-
?
1.1.1.236
N-iso-propylnortropinone + NADPH
-
347973
Datura stramonium
? + NADP+
-
-
-
?
1.1.1.236
N-methyl-4-piperidone + NADPH
-
347973
Datura stramonium
N-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.236
N-methyl-4-piperidone + NADPH
-
347973
Hyoscyamus niger
N-methylpiperidin-4-ol + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
347973
Datura stramonium
pseudotropine + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
347973
Hyoscyamus niger
pseudotropine + NADP+
-
-
-
?
1.1.1.236
tropinone + NADPH + H+
-
347973
Brugmansia x candida
pseudotropine + NADP+
-
-
-
?
1.4.3.22
1,4-diaminobutane + H2O + O2
56% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
4-aminobutanal + NH3 + H2O2
-
-
-
?
1.4.3.22
cadaverine + H2O + O2
15% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
5-aminopentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-(2-fluoroethyl)putrescine + H2O + O2
same activity as with N-methylputrescine as substrate
347973
Brugmansia x candida
N-(2-fluoroethyl)aminobutanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-ethylcadaverine + H2O + O2
17% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
5-(ethylamino)pentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-ethylputrescine + H2O + O2
same activity as with N-methylputrescine as substrate
347973
Brugmansia x candida
4-(ethylamino)butanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-methylcadaverine + H2O + O2
16% activity compared to activity with N-methylputrescine
347973
Brugmansia x candida
5-(methylamino)pentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-methylputrescine + H2O + O2
best substrate
347973
Brugmansia x candida
4-(methylamino)butanal + NH3 + H2O2
-
-
-
?
1.4.3.22
N-n-propylputrescine + H2O + O2
50% of the activity compared to N-methylputrescine as substrate
347973
Brugmansia x candida
4-propylaminobutanal + NH3 + H2O2
-
-
-
?
2.3.1.185
acetyl-CoA + N-ethylnortropan-3-ol
16% of the activity with acetyl-CoA and tropine
347973
Brugmansia x candida
CoA + 8-ethyl-8-azabicyclo[3.2.1]oct-3-yl-acetate
-
-
-
?
2.3.1.185
acetyl-CoA + tropine
-
347973
Brugmansia x candida
CoA + O-acetyltropine
-
-
-
?
2.3.1.185
additional information
no activity with pseudotropine
347973
Brugmansia x candida
?
-
-
-
?
2.3.1.185
tigloyl-CoA + N-ethylnortropan-3-ol
76% of the activity with tigloyl-CoA and tropine
347973
Brugmansia x candida
CoA + 8-ethyl-8-azabicyclo[3.2.1]oct-3-yl (2E)-2-methylbut-2-enoate
-
-
-
?
2.3.1.185
tigloyl-CoA + norpseudopelletierin-3-ol
5% of the activity with tigloyl-CoA and tropine
347973
Brugmansia x candida
CoA + ?
-
-
-
?
2.3.1.185
tigloyl-CoA + tropine
-
347973
Brugmansia x candida
CoA + O-tigloyltropine
-
-
-
?
2.3.1.186
acetyl-CoA + N-(2-fluoroethyl)nortropan-3-ol
16% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-(2-fluoroethyl)nortropan-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + N-ethylnorpseudopelletierin-3-ol
% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-ethylnorpseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + N-ethylnortropan-3-ol
35% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-ethylnortropan-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + N-iso-propylnorpseudopelletierin-3-ol
7% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + N-iso-propylnorpseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + norpseudopelletierin-3-ol
9% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + norpseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + pseudopelletierin-3-ol
% of the activity with acetyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + pseudopelletierin-3-ol acetyl ester
-
-
-
?
2.3.1.186
acetyl-CoA + pseudotropine
-
347973
Brugmansia x candida
CoA + O-acetylpseudotropine
-
-
-
?
2.3.1.186
additional information
no activity with tropine
347973
Brugmansia x candida
?
-
-
-
?
2.3.1.186
tigloyl-CoA + N-(2-fluoroethyl)nortropan-3-ol
74% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-(2-fluoroethyl)nortropan-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + N-ethylnorpseudopelletierin-3-ol
% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-ethylnorpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + N-ethylnortropan-3-ol
81% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-ethylnortropan-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + N-iso-propylnorpseudopelletierin-3-ol
2% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-N-iso-propylnorpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + norpseudopelletierin-3-ol
13% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-norpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + norpseudopelletierin-3-ol
40% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-norpseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + pseudopelletierin-3-ol
% of the activity with tigloyl-CoA and pseudotropine
347973
Brugmansia x candida
CoA + 3-O-tigloyl-pseudopelletierin-3-ol
-
-
-
?
2.3.1.186
tigloyl-CoA + pseudotropine
-
347973
Brugmansia x candida
CoA + O-tigloylpseudotropine
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.4.3.22
physiological function
study of the enzymes of N-alkyltropane biosynthesis: tropinone reductase I (EC 1.1.1.206), tropinone reductase II (EC 1.1.1.236), and tropin:acyl-CoA transferase, pseudotropine:acyl-CoA transferase
Brugmansia x candida
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.4.3.22
physiological function
study of the enzymes of N-alkyltropane biosynthesis: tropinone reductase I (EC 1.1.1.206), tropinone reductase II (EC 1.1.1.236), and tropin:acyl-CoA transferase, pseudotropine:acyl-CoA transferase
Brugmansia x candida