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Literature summary extracted from

  • Bartholomew, B.A.; Smith, M.J.; Long, M.T.; Darcy, P.J.; Trudgill P.W.; Hopper, K.J.
    Tropine dehydrogenase: purification, some properties and an evaluation of its role in the bacterial metabolism of tropine (1995), Biochem. J., 307, 603-608.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.206 0.006
-
 Tropine
-
 Pseudomonas sp.
1.1.1.206 0.048
-
 NADP+
-
 Pseudomonas sp.
1.1.1.206 0.073
-
 nortropine
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.206 28000
-
 SDS-PAGE Pseudomonas sp.
1.1.1.206 58000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.206 tropine + NADP+ Pseudomonas sp. role in the pathway for tropine catabolism tropinone + NADPH
-
 r
1.1.1.206 tropine + NADP+ Pseudomonas sp. AT3 role in the pathway for tropine catabolism tropinone + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.206 Pseudomonas sp.
-
 AT3
-
1.1.1.206 Pseudomonas sp. AT3
-
 AT3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.206
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.206 0.316
-
 crude extract Pseudomonas sp.
1.1.1.206 63.82
-
 purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.206 nortropine + NADP+
-
 Pseudomonas sp. nortropinone + NADPH
-
 ?
1.1.1.206 nortropine + NADP+
-
 Pseudomonas sp. AT3 nortropinone + NADPH
-
 ?
1.1.1.206 tropine + NADP+
-
 Pseudomonas sp. tropinone + NADPH
-
 r
1.1.1.206 tropine + NADP+ role in the pathway for tropine catabolism Pseudomonas sp. tropinone + NADPH
-
 r
1.1.1.206 tropine + NADP+
-
 Pseudomonas sp. AT3 tropinone + NADPH
-
 r
1.1.1.206 tropine + NADP+ role in the pathway for tropine catabolism Pseudomonas sp. AT3 tropinone + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.206 dimer 2 identical subunits, gel filtration Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.206 4
-
 loss of 65% activity after 60 hours in crude extracts Pseudomonas sp.
1.1.1.206 4
-
 loss of 90% activity of the purified enzyme after 10 hours, presence of 10% ethanol during the purification procedure Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.206 additional information no activity with NAD+ Pseudomonas sp.
1.1.1.206 NADP+
-
 Pseudomonas sp.