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Literature summary extracted from

  • Okada, M.; Shimura, K.; Shiraki, H.; Nakagawa, H.
    IMP dehydrogenase. II. Purification and properties of the enzyme from Yoshida sarcoma ascites tumor cells (1983), J. Biochem., 94, 1605-1613.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.205 6-Chloro-9-beta-D-ribofuranosylpurine 5 '-phosphate
-
 Rattus norvegicus
1.1.1.205 AMP
-
 Rattus norvegicus
1.1.1.205 GMP
-
 Rattus norvegicus
1.1.1.205 NAD+
-
 Rattus norvegicus
1.1.1.205 p-chloromercuribenzoate inhibition can be restored by addition of DTT Rattus norvegicus
1.1.1.205 xanthosine 5'-phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.205 additional information
-
 additional information
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.205 K+ full activity in presence of K+ Rattus norvegicus
1.1.1.205 Na+ can partially replace K+ in activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.205 additional information
-
 enzyme tends to aggregate owing to its own physicochemical characteristics Rattus norvegicus
1.1.1.205 68000
-
 2 * 68000, Yoshida sarcoma ascites tumor cells, SDS-PAGE Rattus norvegicus
1.1.1.205 127000
-
 Yoshida sarcoma ascites tumor cells, gel filtration in presence of 10% (NH4)2SO4 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.205 Rattus norvegicus
-
 Yoshida sarcoma ascites cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.205 homogeneitiy Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.205 Yoshida ascites sarcoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.205 1.19
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.205 inosine 5'-phosphate + NAD+ + H2O
-
 Rattus norvegicus xanthosine 5'-phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.205 dimer 2 * 68000, Yoshida sarcoma ascites tumor cells, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.205 7.8 8
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.205 6.7 9 pH 6.7: about 40% of activity maximum, pH 9.0: about 55% of activity maximum Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.205 NAD+
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.205 0.109
-
 xanthosine 5'-phosphate
-
 Rattus norvegicus
1.1.1.205 0.13
-
 GMP
-
 Rattus norvegicus
1.1.1.205 0.854
-
 AMP
-
 Rattus norvegicus