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Literature summary extracted from

  • Negm, F.B.; Loescher, W.H.
    Characterization and partial purification of aldose-6-phosphate reductase (alditol-6-phosphate:NADP 1-oxidoreductase) from apple leaves (1981), Plant Physiol., 67, 139-142.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.200 AgNO3 complete inhibition at 0.1 mM Malus domestica
1.1.1.200 cysteine 47% inhibition of oxidation and 11% inhibition of reduction at 10 mM Malus domestica
1.1.1.200 iodoacetate 42% inhibition of oxidation and 19% inhibition of reduction at 10 mM Malus domestica
1.1.1.200 Na2EDTA 35% inhibition of oxidation and 11% inhibition of reduction at 10 mM Malus domestica
1.1.1.200 p-chloromercuribenzoate complete inhibition at 1.0 mM Malus domestica
1.1.1.200 ZnSO4 complete inhibition of oxidation and 80% inhibition of reduction at 1.25 mM Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.200 0.2
-
 D-glucose 6-phosphate
-
 Malus domestica
1.1.1.200 3.9
-
 D-sorbitol 6-phosphate
-
 Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.200 additional information Prunus armeniaca important role in sorbitol synthesis ?
-
 ?
1.1.1.200 additional information Prunus persica important role in sorbitol synthesis ?
-
 ?
1.1.1.200 additional information Malus domestica important role in sorbitol synthesis ?
-
 ?
1.1.1.200 additional information Pyrus communis important role in sorbitol synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.200 Malus domestica
-
 apple, cv. Starkrimson
-
1.1.1.200 Malus domestica
-
 cv. Antonovka
-
1.1.1.200 Malus domestica
-
 cv. Golden Delicious
-
1.1.1.200 Prunus armeniaca
-
 apricot, cv. Perfection
-
1.1.1.200 Prunus persica
-
 peach, cv. Redhaven
-
1.1.1.200 Pyrus communis
-
 pear, cv. Conference, cv. Bartlett
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.200
-
 Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.200 leaf photosynthetically active tissue Prunus armeniaca
-
1.1.1.200 leaf photosynthetically active tissue Prunus persica
-
1.1.1.200 leaf photosynthetically active tissue Malus domestica
-
1.1.1.200 leaf photosynthetically active tissue Pyrus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.200 additional information
-
 measurement during development Malus domestica
1.1.1.200 additional information
-
 distribution in Rosaceae species Prunus armeniaca
1.1.1.200 additional information
-
 distribution in Rosaceae species Prunus persica
1.1.1.200 additional information
-
 distribution in Rosaceae species Malus domestica
1.1.1.200 additional information
-
 distribution in Rosaceae species Pyrus communis
1.1.1.200 628
-
-
 Malus domestica

Storage Stability

EC Number Storage Stability Organism
1.1.1.200 1°C, 1 week, partially purified enzyme Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.200 2-deoxy-D-glucose 6-phosphate + NADPH reduced at low rate Malus domestica ?
-
 ?
1.1.1.200 D-galactose 6-phosphate + NADPH reduced at a higher rate than D-glucose 6-phosphate Malus domestica ?
-
 r
1.1.1.200 D-mannose 6-phosphate + NADPH reduced at low rate Malus domestica ?
-
 ?
1.1.1.200 D-sorbitol 6-phosphate + NADP+
-
 Prunus armeniaca D-glucose 6-phosphate + NADPH + H+
-
 r
1.1.1.200 D-sorbitol 6-phosphate + NADP+
-
 Prunus persica D-glucose 6-phosphate + NADPH + H+
-
 r
1.1.1.200 D-sorbitol 6-phosphate + NADP+
-
 Malus domestica D-glucose 6-phosphate + NADPH + H+
-
 r
1.1.1.200 D-sorbitol 6-phosphate + NADP+
-
 Pyrus communis D-glucose 6-phosphate + NADPH + H+
-
 r
1.1.1.200 additional information not: D-glucose 1-phosphate, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose, sorbitol Malus domestica ?
-
 ?
1.1.1.200 additional information important role in sorbitol synthesis Prunus armeniaca ?
-
 ?
1.1.1.200 additional information important role in sorbitol synthesis Prunus persica ?
-
 ?
1.1.1.200 additional information important role in sorbitol synthesis Malus domestica ?
-
 ?
1.1.1.200 additional information important role in sorbitol synthesis Pyrus communis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.200 30 35 D-sorbitol 6-phosphate oxidation Malus domestica
1.1.1.200 35
-
 D-glucose 6-phosphate reduction Malus domestica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.200 additional information
-
 no activity at 50°C Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.200 9.5
-
 both directions Malus domestica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.200 8.5 10.1 about 50% of activity maximum at pH 8.5 and 10.1 Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.200 additional information no activity with NAD+ or NADH Malus domestica
1.1.1.200 NADP+
-
 Malus domestica
1.1.1.200 NADPH
-
 Malus domestica