BRENDA - Enzyme Database

An NADP-linked 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin D2 from swine brain

Tokumoto, H.; Watanabe, K.; Fukushima, D.; Shimizu, T.; Hayaishi, O.; J. Biol. Chem. 257, 13576-13580 (1982) View publication on PubMed

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.196
unstable
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.196
11,14-Eicosadienoic acid
-
Sus scrofa
1.1.1.196
3,3',5-Triiodothyroacetic acid
-
Sus scrofa
1.1.1.196
arachidonic acid
-
Sus scrofa
1.1.1.196
Flufenamic acid
-
Sus scrofa
1.1.1.196
indomethacin
-
Sus scrofa
1.1.1.196
L-thyroxine
-
Sus scrofa
1.1.1.196
linoleic acid
-
Sus scrofa
1.1.1.196
N-ethylmaleimide
-
Sus scrofa
1.1.1.196
p-chloromercuribenzoate
-
Sus scrofa
1.1.1.196
palmitic acid
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.196
0.0004
-
NADP+
-
Sus scrofa
1.1.1.196
0.052
-
Prostaglandin D2
-
Sus scrofa
1.1.1.196
0.278
-
prostaglandin D1
-
Sus scrofa
1.1.1.196
0.35
-
prostaglandin E2
-
Sus scrofa
1.1.1.196
0.4
-
prostaglandin F2alpha
-
Sus scrofa
1.1.1.196
0.54
-
prostaglandin I2
-
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.196
28000
-
gel electrophoresis
Sus scrofa
1.1.1.196
29000
-
1 * 29000
Sus scrofa
1.1.1.196
30000
-
sedimentation equilibrium
Sus scrofa
1.1.1.196
32000
-
gel filtration
Sus scrofa
1.1.1.196
52000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.196
prostaglandin D2 + NADP+
Homo sapiens
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
Sus scrofa
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.196
Homo sapiens
-
human
-
1.1.1.196
Sus scrofa
-
swine
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.196
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.196
blood
platelet
Homo sapiens
-
1.1.1.196
brain
-
Homo sapiens
-
1.1.1.196
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.196
0.0212
-
-
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.196
4°C, at pH 7 for 6 h, 54% loss of enzyme activity
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.196
prostaglandin D1 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D1 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
-
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin E2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin E2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin F2alpha + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin F2alpha + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin I2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin I2 + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.196
monomer
1 * 28000, SDS-PAGE
Sus scrofa
1.1.1.196
monomer
1 * 29000
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.196
9.5
-
optimal for reaction with prostaglandins D1, D2, E2 and I2
Sus scrofa
1.1.1.196
9.5
10
with prostaglandin F2alpha as substrate
Sus scrofa
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.196
7
-
most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9
Sus scrofa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.196
NADP+
strict specificity for
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.196
NADP+
strict specificity for
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.196
unstable
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.196
11,14-Eicosadienoic acid
-
Sus scrofa
1.1.1.196
3,3',5-Triiodothyroacetic acid
-
Sus scrofa
1.1.1.196
arachidonic acid
-
Sus scrofa
1.1.1.196
Flufenamic acid
-
Sus scrofa
1.1.1.196
indomethacin
-
Sus scrofa
1.1.1.196
L-thyroxine
-
Sus scrofa
1.1.1.196
linoleic acid
-
Sus scrofa
1.1.1.196
N-ethylmaleimide
-
Sus scrofa
1.1.1.196
p-chloromercuribenzoate
-
Sus scrofa
1.1.1.196
palmitic acid
-
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.196
0.0004
-
NADP+
-
Sus scrofa
1.1.1.196
0.052
-
Prostaglandin D2
-
Sus scrofa
1.1.1.196
0.278
-
prostaglandin D1
-
Sus scrofa
1.1.1.196
0.35
-
prostaglandin E2
-
Sus scrofa
1.1.1.196
0.4
-
prostaglandin F2alpha
-
Sus scrofa
1.1.1.196
0.54
-
prostaglandin I2
-
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.196
28000
-
gel electrophoresis
Sus scrofa
1.1.1.196
29000
-
1 * 29000
Sus scrofa
1.1.1.196
30000
-
sedimentation equilibrium
Sus scrofa
1.1.1.196
32000
-
gel filtration
Sus scrofa
1.1.1.196
52000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.196
prostaglandin D2 + NADP+
Homo sapiens
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
Sus scrofa
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.196
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.196
blood
platelet
Homo sapiens
-
1.1.1.196
brain
-
Homo sapiens
-
1.1.1.196
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.196
0.0212
-
-
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.196
4°C, at pH 7 for 6 h, 54% loss of enzyme activity
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.196
prostaglandin D1 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D1 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
-
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin E2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin E2 + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin F2alpha + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin F2alpha + NADPH + H+
-
-
-
?
1.1.1.196
prostaglandin I2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin I2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.196
monomer
1 * 28000, SDS-PAGE
Sus scrofa
1.1.1.196
monomer
1 * 29000
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.196
9.5
-
optimal for reaction with prostaglandins D1, D2, E2 and I2
Sus scrofa
1.1.1.196
9.5
10
with prostaglandin F2alpha as substrate
Sus scrofa
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.196
7
-
most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9
Sus scrofa