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Literature summary extracted from

  • MacDonald, I.A.; Meier, E.C.; Mahony, D.E.; Costain, G.A.
    3alpha-, 7alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Clostridium perfringens (1976), Biochim. Biophys. Acta, 450, 142-153.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.176 0.8
-
 3alpha,12alpha-dihydroxy-5beta-cholanoate
-
 Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.176 Clostridium perfringens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.176 cholic acid + NAD(P)+
-
 Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Clostridium perfringens 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.176 taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.176 43
-
 slow inactivation Clostridium perfringens
1.1.1.176 56
-
 rapid inactivation Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.176 10.5
-
 oxidation of bile acids Clostridium perfringens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.176 NAD+
-
 Clostridium perfringens