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Literature summary extracted from

  • MacDonald, I.A.; Mahony, D.E.; Jellet, J.F.; Meier, C.E.
    NAD-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Eubacterium lentum ATCC no. 25559 (1977), Biochim. Biophys. Acta, 489, 466-476.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.002 0.0024 17beta-hydroxy-5alpha-androstan-3-one also in this range: 3alpha,7alpha-dihydroxy-5beta-cholanoyl glycine Eggerthella lenta
1.1.1.50 0.007 0.0083 5alpha-dihydrotestosterone also in this range: 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine Eggerthella lenta
1.1.1.50 0.01 0.025 cholic acid NAD+, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine Eggerthella lenta
1.1.1.176 0.024
-
 NAD+
-
 Eggerthella lenta
1.1.1.176 0.1
-
 7alpha,12alpha-dihydroxy-5beta-cholanoate
-
 Eggerthella lenta

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Eggerthella lenta
-
-
-
1.1.1.176 Eggerthella lenta
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.50 4°C, 2 days Eggerthella lenta
1.1.1.176 -20°C, lyophilized, 4 months Eggerthella lenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine + NAD+
-
 Eggerthella lenta 12alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.50 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine + NAD+
-
 Eggerthella lenta 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl glycine + NADH
-
 ?
1.1.1.50 3alpha,7alpha-dihydroxy-5beta-cholanoyl glycine + NAD+
-
 Eggerthella lenta 7alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.50 3alpha-hydroxy-5beta-androstan-17-one + NADP+
-
 Eggerthella lenta 5beta-androstan-3,17-dione + NADPH
-
 r
1.1.1.50 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 Eggerthella lenta 17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
 r
1.1.1.50 5beta-androstan-3alpha,17beta-diol + NADP+
-
 Eggerthella lenta 17beta-hydroxy-5beta-androstan-3-one + NADPH
-
 r
1.1.1.50 androsterone + NAD(P)+ i.e. 3alpha-hydroxy-5alpha-androstan-17-one Eggerthella lenta 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 cholic acid + NAD+
-
 Eggerthella lenta 3-oxocholic acid + NADH + H+
-
 r
1.1.1.176 7alpha,12alpha-dihydroxy-5beta-cholanoate + NAD+
-
 Eggerthella lenta 7alpha-hydroxy-12-oxo-5beta-cholanoate + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.50 50
-
 30-60 min stable Eggerthella lenta
1.1.1.176 50
-
 30 min, inactivation Eggerthella lenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 11.4
-
-
 Eggerthella lenta
1.1.1.176 8.5 10.5 oxidation of bile acids Eggerthella lenta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.176 NAD+
-
 Eggerthella lenta
1.1.1.176 NADP+ low activity Eggerthella lenta