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Literature summary extracted from

  • Harris, J.N.; Hylemon, P.B.
    Partial purification and characterization of NADP-dependent 12alpha-hydroxysteroid dehydrogenase from Clostridium leptum (1978), Biochim. Biophys. Acta, 528, 148-157.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.176 0.44
-
 3alpha,12alpha-dihydroxy-5beta-cholanoyl taurine
-
 [Clostridium] leptum
1.1.1.176 0.57
-
 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
-
 [Clostridium] leptum
1.1.1.176 1.6
-
 3alpha,12alpha-dihydroxy-5beta-cholanoate
-
 [Clostridium] leptum
1.1.1.176 2.7
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
 [Clostridium] leptum
1.1.1.176 4
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine
-
 [Clostridium] leptum
1.1.1.176 7
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
 [Clostridium] leptum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.176 225000
-
 gel filtration [Clostridium] leptum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.176 [Clostridium] leptum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.176
-
 [Clostridium] leptum

Storage Stability

EC Number Storage Stability Organism
1.1.1.176 -20°C, 20 days, 50% loss of activity [Clostridium] leptum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.176 cholic acid + NAD(P)+
-
 [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.176 glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.176 taurocholic acid + NAD(P)+
-
 [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
 ?
1.1.1.176 taurodeoxycholic acid + NAD+
-
 [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.176
-
 4 half-life: 11 days [Clostridium] leptum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.176 8.5 9 oxidation of cholic acid [Clostridium] leptum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.176 NADP+
-
 [Clostridium] leptum