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Literature summary extracted from

  • MacDonald, I.A.; Jellett, J.F.; Mahony, D.E.; Holdeman, L.V.
    Bile salt 3alpha- and 12alpha-hydroxysteroid dehydrogenases from Eubacterium lentum and related organisms (1979), Appl. Environ. Microbiol., 37, 992-1000.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.176 0.028
-
 3alpha,12alpha-dihydroxy-5beta-cholanoate
-
 Eggerthella lenta
1.1.1.176 0.03
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl methyl ester 3alpha,12alpha-dihydroxy-5beta-cholanoate Eggerthella lenta
1.1.1.176 0.059
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
 Eggerthella lenta
1.1.1.176 0.17
-
 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
-
 Eggerthella lenta
1.1.1.176 0.25
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
 Eggerthella lenta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 205000
-
 gel filtration Eggerthella lenta
1.1.1.176 125000
-
 gel filtration Eggerthella lenta

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Eggerthella lenta
-
-
-
1.1.1.176 Eggerthella lenta
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.176 -20°C, 3 months, 5% loss of activity Eggerthella lenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.176 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl methyl ester + NADP+
-
 Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl methyl ester + NADPH + H+
-
 r
1.1.1.176 allocholate + NAD+
-
 Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
-
 ?
1.1.1.176 allodeoxycholate + NAD+
-
 Eggerthella lenta 3alpha-hydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
-
 ?
1.1.1.176 cholic acid + NAD(P)+
-
 Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Eggerthella lenta 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.176 glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine Eggerthella lenta 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.176 taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.176 8 10.5 oxidation of cholic acid Eggerthella lenta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.176 8 10.5
-
 Eggerthella lenta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.176 NAD+
-
 Eggerthella lenta