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Literature summary extracted from

  • Jones, J.P.; O'Hare, E.J.; Wong, L.L.
    Oxidation of polychlorinated benzenes by genetically engineered CYP101 (cytochrome P450(cam)) (2001), Eur. J. Biochem., 268, 1460-1467.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 V247L increased turnover rate for NADH Pseudomonas putida
1.14.15.1 Y96F 100fold increase of activity Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
 Accession Number PC00183
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 1,2,4,5-tetrachlorobenzene + putidaredoxin + O2
-
 Pseudomonas putida 2,3,5,6-tetrachlorophenol + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1,2-dichlorobenzene + putidaredoxin + O2
-
 Pseudomonas putida 2,3-dichlorophenol + 3,4-dichlorophenol + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1,3,5-trichlorobenzene + putidaredoxin + O2
-
 Pseudomonas putida 2,4,6-trichlorophenol + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1,3-dichlorobenzene + putidaredoxin + O2
-
 Pseudomonas putida 2,6-dichlorophenol + 2,4-dichlorophenol + 2,5-dichlorophenol + 2,3-dichlorophenol + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1,4-dichlorobenzene + putidaredoxin + O2
-
 Pseudomonas putida 2,5-dichlorophenol + oxidized putidaredoxin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 NADH
-
 Pseudomonas putida