BRENDA - Enzyme Database show

Oxidation of polychlorinated benzenes by genetically engineered CYP101 (cytochrome P450(cam))

Jones, J.P.; O'Hare, E.J.; Wong, L.L.; Eur. J. Biochem. 268, 1460-1467 (2001)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.1
V247L
increased turnover rate for NADH
Pseudomonas putida
1.14.15.1
Y96F
100fold increase of activity
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.1
(+)-camphor + putidaredoxin + O2
Pseudomonas putida
-
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
i.e. (+)-exo-5-hydroxycamphor
Pseudomonas putida
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.1
Pseudomonas putida
-
Accession Number PC00183
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.1
(+)-camphor + putidaredoxin + O2
-
347708
Pseudomonas putida
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
i.e. (+)-exo-5-hydroxycamphor
347708
Pseudomonas putida
-
1.14.15.1
(+)-camphor + putidaredoxin + O2
-
347708
Pseudomonas putida
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
i.e. (+)-exo-5-hydroxycamphor
347708
Pseudomonas putida
?
1.14.15.1
1,2,4,5-tetrachlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,3,5,6-tetrachlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,2-dichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,3-dichlorophenol + 3,4-dichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,3,5-trichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,4,6-trichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,3-dichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,6-dichlorophenol + 2,4-dichlorophenol + 2,5-dichlorophenol + 2,3-dichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,4-dichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,5-dichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.1
NADH
-
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.1
NADH
-
Pseudomonas putida
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.1
V247L
increased turnover rate for NADH
Pseudomonas putida
1.14.15.1
Y96F
100fold increase of activity
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.1
(+)-camphor + putidaredoxin + O2
Pseudomonas putida
-
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
i.e. (+)-exo-5-hydroxycamphor
Pseudomonas putida
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.1
(+)-camphor + putidaredoxin + O2
-
347708
Pseudomonas putida
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
i.e. (+)-exo-5-hydroxycamphor
347708
Pseudomonas putida
-
1.14.15.1
(+)-camphor + putidaredoxin + O2
-
347708
Pseudomonas putida
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
i.e. (+)-exo-5-hydroxycamphor
347708
Pseudomonas putida
?
1.14.15.1
1,2,4,5-tetrachlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,3,5,6-tetrachlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,2-dichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,3-dichlorophenol + 3,4-dichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,3,5-trichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,4,6-trichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,3-dichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,6-dichlorophenol + 2,4-dichlorophenol + 2,5-dichlorophenol + 2,3-dichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?
1.14.15.1
1,4-dichlorobenzene + putidaredoxin + O2
-
347708
Pseudomonas putida
2,5-dichlorophenol + oxidized putidaredoxin + H2O
-
347708
Pseudomonas putida
?