Literature summary extracted from

Burnell, J.N.; Whatley, F.R.
Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of serine transacetylase, O-acetylserine sulphydrylase and beta-cystathionase (1977), Biochim. Biophys. Acta, 481, 246-265.

Inhibitors

EC Number	Inhibitors	Comment	Organism
2.3.1.30	CoA	weak	Paracoccus denitrificans
2.3.1.30	D-cysteine	-	Paracoccus denitrificans
2.3.1.30	hydroxylamine	-	Paracoccus denitrificans
2.3.1.30	iodoacetamide	-	Paracoccus denitrificans
2.3.1.30	KCN	-	Paracoccus denitrificans
2.3.1.30	L-cysteine	-	Paracoccus denitrificans
2.3.1.30	L-homocysteine	weak	Paracoccus denitrificans
2.3.1.30	L-homoserine	weak	Paracoccus denitrificans
2.3.1.30	L-methionine	-	Paracoccus denitrificans
2.3.1.30	N-ethylmaleimide	-	Paracoccus denitrificans
2.3.1.30	p-chloromercuribenzoate	-	Paracoccus denitrificans
2.5.1.47	cystathionine	competitive to sulfide	Paracoccus denitrificans
2.5.1.47	L-homocysteine	competitive to sulfide	Paracoccus denitrificans
2.5.1.47	methionine	competitive to sulfide	Paracoccus denitrificans
2.5.1.47	serine	competitive to O-acetylserine	Paracoccus denitrificans
2.5.1.47	SO32-	competitive to sulfide	Paracoccus denitrificans
4.4.1.13	Cys	strong	Paracoccus denitrificans
4.4.1.13	glutathione	strong	Paracoccus denitrificans
4.4.1.13	homocysteine	strong	Paracoccus denitrificans
4.4.1.13	hydroxylamine	-	Paracoccus denitrificans
4.4.1.13	iodoacetamide	-	Paracoccus denitrificans
4.4.1.13	KCN	-	Paracoccus denitrificans
4.4.1.13	NaF	10 mM, weak	Paracoccus denitrificans
4.4.1.13	NEM	-	Paracoccus denitrificans
4.4.1.13	O-acetylserine	strong	Paracoccus denitrificans
4.4.1.13	PCMB	-	Paracoccus denitrificans
4.4.1.13	phenylhydrazine	-	Paracoccus denitrificans

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
2.3.1.30	0.1	-	acetyl-CoA	-	Paracoccus denitrificans
2.3.1.30	0.4	-	L-serine	-	Paracoccus denitrificans
2.5.1.47	1.25	-	H2S	-	Paracoccus denitrificans
2.5.1.47	2.7	-	O-acetyl-L-Ser	pH 7.5, 30°C	Paracoccus denitrificans
4.4.1.13	4.2	-	cystathionine	-	Paracoccus denitrificans

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.5.1.47	soluble	-	Paracoccus denitrificans	-	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.1.30	acetyl-CoA + L-serine	Paracoccus denitrificans	enzyme in metabolic pathway of sulfur from sulfide to methionine	CoA + O-acetyl-L-serine	-	ir
2.5.1.47	O-acetyl-L-Ser + sulfide	Paracoccus denitrificans	controlled by feedback inhibition, adaptively significant as sulfide removal mechanism	L-Cys + acetate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.30	Paracoccus denitrificans	-	-	-
2.3.1.30	Paracoccus denitrificans 8944	-	-	-
2.5.1.47	Paracoccus denitrificans	-	-	-
4.4.1.13	Paracoccus denitrificans	-	-	-
4.4.1.13	Paracoccus denitrificans 8944	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.1.30	partially	Paracoccus denitrificans
2.5.1.47	-	Paracoccus denitrificans
4.4.1.13	-	Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.5.1.47	additional information	-	activity increased during growth on cystine, as compared to growth on sulfate	Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.3.1.30	acetyl-CoA + L-serine	specific for L-serine	Paracoccus denitrificans	CoA + O-acetyl-L-serine	-	ir
2.3.1.30	acetyl-CoA + L-serine	enzyme in metabolic pathway of sulfur from sulfide to methionine	Paracoccus denitrificans	CoA + O-acetyl-L-serine	-	ir
2.5.1.47	O-acetyl-L-Ser + sulfide	controlled by feedback inhibition, adaptively significant as sulfide removal mechanism	Paracoccus denitrificans	L-Cys + acetate	-	?
2.5.1.47	O-acetyl-L-serine	-	Paracoccus denitrificans	L-cysteine + acetate	-	?
2.5.1.47	O-acetyl-Ser + selenide	maximal 40% rate of cysteine synthesis	Paracoccus denitrificans	selenocysteine + acetate	-	?
4.4.1.13	L-cystathionine + H2O	-	Paracoccus denitrificans	L-homocysteine + NH3 + pyruvate	-	?
4.4.1.13	L-cystathionine + H2O	-	Paracoccus denitrificans 8944	L-homocysteine + NH3 + pyruvate	-	?
4.4.1.13	L-cystine + H2O	28% of the activity with L-cystathionine	Paracoccus denitrificans	L-thiocysteine + pyruvate + NH3	-	?
4.4.1.13	L-cystine + H2O	28% of the activity with L-cystathionine	Paracoccus denitrificans 8944	L-thiocysteine + pyruvate + NH3	-	?
4.4.1.13	L-djenkolate + H2O	79% of the activity with L-cystathionine	Paracoccus denitrificans	pyruvate	-	?
4.4.1.13	L-djenkolate + H2O	79% of the activity with L-cystathionine	Paracoccus denitrificans 8944	pyruvate	-	?
4.4.1.13	L-homocysteine + H2O	5% of the activity with L-cystathionine	Paracoccus denitrificans	?	-	?
4.4.1.13	L-homocysteine + H2O	5% of the activity with L-cystathionine	Paracoccus denitrificans 8944	?	-	?
4.4.1.13	lanthionine + H2O	DL-lanthionine, 136% of the activity with L-cystathionine	Paracoccus denitrificans	L-cysteine + NH3 + pyruvate	-	?
4.4.1.13	lanthionine + H2O	DL-lanthionine, 136% of the activity with L-cystathionine	Paracoccus denitrificans 8944	L-cysteine + NH3 + pyruvate	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.1.30	30	-	assay at	Paracoccus denitrificans

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2.5.1.47	80	-	3 min, unstable	Paracoccus denitrificans

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.1.30	7.5	8	-	Paracoccus denitrificans
2.5.1.47	7.5	-	-	Paracoccus denitrificans
4.4.1.13	9	9.5	-	Paracoccus denitrificans

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.5.1.47	pyridoxal 5'-phosphate	-	Paracoccus denitrificans
4.4.1.13	pyridoxal 5'-phosphate	required as cofactor	Paracoccus denitrificans