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Literature summary extracted from

  • Dwivedi, C.M.; Ragin, R.C.; Uren, J.R.
    Cloning, purification, and characterization of beta-cystathionase from Escherichia coli (1982), Biochemistry, 21, 3064-3069.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13
-
 Escherichia coli

General Stability

EC Number General Stability Organism
4.4.1.13 not stable to lyophilization Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 3,3,3,-trifluoroalanine irreversible Escherichia coli
4.4.1.13 Beta-cyanoalanine
-
 Escherichia coli
4.4.1.13 Cys competitive with L-cystathionine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.04
-
 L-cystathionine
-
 Escherichia coli
4.4.1.13 0.25
-
 L-cystine
-
 Escherichia coli
4.4.1.13 0.36
-
 L-djenkolate
-
 Escherichia coli
4.4.1.13 0.83
-
 L-meso-lanthionine
-
 Escherichia coli
4.4.1.13 4.5
-
 L-homolanthionine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 280000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 215
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -20°C or 4°C, 50% glycerol, 4 months, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cystathionine + H2O
-
 Escherichia coli L-homocysteine + NH3 + pyruvate
-
 ?
4.4.1.13 L-cysteine + H2O
-
 Escherichia coli ?
-
 ?
4.4.1.13 L-cystine + H2O
-
 Escherichia coli L-thiocysteine + pyruvate + NH3
-
 ?
4.4.1.13 L-djenkolate + H2O
-
 Escherichia coli pyruvate
-
 ?
4.4.1.13 L-homolanthionine + H2O
-
 Escherichia coli ?
-
 ?
4.4.1.13 L-meso-lanthionine + H2O
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 hexamer
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8 9
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.13 7.2 9 pH 7.2: 36% of maximal activity, pH 8.0-9.0: optimum Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate 5.7 mol of pyridoxal 5'-phosphate is bound per mol of enzyme Escherichia coli
4.4.1.13 pyridoxal 5'-phosphate Km: 0.005 mM Escherichia coli