BRENDA - Enzyme Database

Purification and characterization of dimethylsulfoniopropionate lyase from an Alcaligenes-like dimethyl sulfide-producing marine isolate

De Souza, M.P.; Yoch, D.C.; Appl. Environ. Microbiol. 61, 21-26 (1995)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
1.4
-
dimethylsulfoniopropionate
-
Alcaligenes sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.4.1.3
48000
-
gel filtration
Alcaligenes sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.4.1.3
additional information
Alcaligenes sp.
inducible by both acrylate and dimethylsulfoniopropionate
?
-
-
-
4.4.1.3
additional information
Alcaligenes sp. M3A
inducible by both acrylate and dimethylsulfoniopropionate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.4.1.3
Alcaligenes sp.
-
-
-
4.4.1.3
Alcaligenes sp. M3A
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.4.1.3
-
Alcaligenes sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.4.1.3
390.1
-
-
Alcaligenes sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
additional information
inducible by both acrylate and dimethylsulfoniopropionate
34605
Alcaligenes sp.
?
-
-
-
-
4.4.1.3
additional information
inducible by both acrylate and dimethylsulfoniopropionate
34605
Alcaligenes sp. M3A
?
-
-
-
-
4.4.1.3
S,S-dimethyl-beta-propiothetin
-
34605
Alcaligenes sp.
dimethyl sulfide + acrylate
-
-
-
-
4.4.1.3
S,S-dimethyl-beta-propiothetin
-
34605
Alcaligenes sp. M3A
dimethyl sulfide + acrylate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.4.1.3
monomer
1 * 48000, SDS-PAGE
Alcaligenes sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.4.1.3
37
40
-
Alcaligenes sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.4.1.3
8
-
-
Alcaligenes sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
1.4
-
dimethylsulfoniopropionate
-
Alcaligenes sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.4.1.3
48000
-
gel filtration
Alcaligenes sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.4.1.3
additional information
Alcaligenes sp.
inducible by both acrylate and dimethylsulfoniopropionate
?
-
-
-
4.4.1.3
additional information
Alcaligenes sp. M3A
inducible by both acrylate and dimethylsulfoniopropionate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.4.1.3
-
Alcaligenes sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.4.1.3
390.1
-
-
Alcaligenes sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
additional information
inducible by both acrylate and dimethylsulfoniopropionate
34605
Alcaligenes sp.
?
-
-
-
-
4.4.1.3
additional information
inducible by both acrylate and dimethylsulfoniopropionate
34605
Alcaligenes sp. M3A
?
-
-
-
-
4.4.1.3
S,S-dimethyl-beta-propiothetin
-
34605
Alcaligenes sp.
dimethyl sulfide + acrylate
-
-
-
-
4.4.1.3
S,S-dimethyl-beta-propiothetin
-
34605
Alcaligenes sp. M3A
dimethyl sulfide + acrylate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.4.1.3
monomer
1 * 48000, SDS-PAGE
Alcaligenes sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.4.1.3
37
40
-
Alcaligenes sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.4.1.3
8
-
-
Alcaligenes sp.