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Literature summary extracted from
- Adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum. Overexpression in Escherichia coli, purification, and characterization of the recombinant enzyme (1994), J. Biol. Chem., 269, 20425-20430.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.1 | overexpression of component E and S in Escherichia coli | Clostridium tetanomorphum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.1 | Clostridium tetanomorphum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.1 | cloning and overexpression in Escherichia coli allows component E to be obtained in homogeneous form, free of inhibiting cobamides and traces of component S | Clostridium tetanomorphum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.1 | additional information | - |
component E | Clostridium tetanomorphum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.1 | -20°C, component E in presence of 50% glycerol, stable for several months | Clostridium tetanomorphum |
| 5.4.99.1 | 4°C or -20°C, component S in concentrated solution in the absence of thiols, stable for several months | Clostridium tetanomorphum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.1 | L-Glu | - |
Clostridium tetanomorphum | threo-3-Methylaspartate | - |
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