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Literature summary extracted from

  • Rauhut, R.; Gabius, H.J.; Cramer, F.
    Purification and properties of phenylalanyl-tRNA synthetase from a higher plant (Phaseolus vulgaris) (1984), Hoppe-Seyler's Z. Physiol. Chem., 365, 289-296.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 0.007
-
 Phe
-
 Phaseolus vulgaris
6.1.1.20 0.08
-
 ATP ATP Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.20 KCl optimal concentration is 10 mM Phaseolus vulgaris
6.1.1.20 Mg2+ optimal concentration is 20 mM Phaseolus vulgaris
6.1.1.20 Mg2+ both MgP2O7- and Mg2P2O7 participate in the pyrophosphorolysis of the aminoacyl adenylate Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.20 59000
-
 2 * 59000 (alpha) + 2 * 70000 (beta), PAGE under denaturing conditions Phaseolus vulgaris
6.1.1.20 70000
-
 2 * 59000 (alpha) + 2 * 70000 (beta), PAGE under denaturing conditions Phaseolus vulgaris
6.1.1.20 260000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.20
-
 Phaseolus vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.20 additional information
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe tRNAs from E. coli and bean chloroplast are not aminoacylated Phaseolus vulgaris AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 tetramer 2 * 59000 (alpha) + 2 * 70000 (beta), PAGE under denaturing conditions Phaseolus vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.20 additional information
-
 additional information
-
 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.20 6.5
-
 2-(morpholino)ethanesulfonic acid buffer or 2-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)aminoethanesulfonic acid buffer Phaseolus vulgaris
6.1.1.20 8.5
-
 Tris/HCl buffer Phaseolus vulgaris