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Literature summary extracted from

  • Alberati-Giani, D.; Malherbe, P.; Köhler, C.; Lang, G.; Kiefer, V.; Lahm, H.W.; Cesura, A.M.
    Cloning and characterization of a soluble kynurenine aminotransferase from rat brain: identity with kidney cysteine conjugate beta-lyase (1995), J. Neurochem., 64, 1448-1455.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.7 transient expression in HEK-293 cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.083
-
 L-kynurenine pH 8.0, 37°C, recombinant enzyme, cosubstrate 2-oxoglutarate Rattus norvegicus
2.6.1.7 1.2
-
 L-kynurenine pH 8.0, 37°C, recombinant enzyme, cosubstrate pyruvate Rattus norvegicus
2.6.1.7 1.7
-
 L-kynurenine pH 8.0, 37°C, cosubstrate pyruvate Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.7 cytosol
-
 Rattus norvegicus 5829
-
2.6.1.7 soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 47000
-
 2 * 47000, SDS-PAGE Rattus norvegicus
2.6.1.7 90000 100000 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Rattus norvegicus
-
 identical to kidney soluble cysteine conjugate beta-lyase also referred to as glutamine transaminase K, K stands for kidney
-
4.4.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 DEAE-Sepharose, phenyl-Sepharose, Superose 12, Mono Q Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.7 brain
-
 Rattus norvegicus
-
2.6.1.7 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.7 0.36
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxoglutarate soluble kidney/brain kynurenine aminotransferase has both amino transferase and cysteine conjugate beta-lyase, i.e., glutamine transaminase K activities Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
 ?
2.6.1.7 L-kynurenine + pyruvate 100fold higher maximal velocity than with 2-oxoglutarate Rattus norvegicus kynurenic acid + L-alanine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 dimer 2 * 47000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.7 KAT
-
 Rattus norvegicus
2.6.1.7 More identical to kidney soluble cysteine conjugate beta-lyase, EC 2.6.1.64, also referred to as glutamine transaminase K, EC 2.6.1.15, K stands for kidney Rattus norvegicus