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Literature summary extracted from

  • Tomisawa, H.; Ichihara, S.; Fukazawa, H.; Ichimoto, N.; Tateishi, M.; Yamamoto, I.
    Purification and characterization of human hepatic cysteine-conjugate beta-lyase (1986), Biochem. J., 235, 569-575.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 hydroxylamine
-
 Homo sapiens
4.4.1.13 KCN
-
 Homo sapiens
4.4.1.13 additional information not: thiol blocking agents, EDTA Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.7
-
 S-(p-bromophenyl)-L-cysteine
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.13 additional information no effect: Ca2+, Zn2+, Mg2+, Fe2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 88000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.13 additional information Homo sapiens detoxification ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.13 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -20°C, 3 months, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 additional information detoxification Homo sapiens ?
-
 ?
4.4.1.13 S-(p-bromophenyl)-L-cysteine
-
 Homo sapiens p-bromobenzenethiol + NH3 + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.13 60
-
 complete loss of activity after 2 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8.5
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.4.1.13 3.2
-
 70% loss of activity after 20 h Homo sapiens
4.4.1.13 4.3
-
 20% loss of activity after 20 h Homo sapiens
4.4.1.13 5.3 8.9 95% of activity retained after 20 h Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate
-
 Homo sapiens