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Literature summary extracted from

  • Barker, H.A.
    Coenzyme B12-dependent mutases causing carbon chain rearrangements (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 6, 509-537.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.4 1-Methyl-1,2-cis-cyclopropanedicarboxylate noncompetitive, slight inhibitor Eubacterium barkeri
5.4.99.4 1-Methyl-1,2-trans-cyclopropanedicarboxylate competitive Eubacterium barkeri
5.4.99.4 glutaconate noncompetitive Eubacterium barkeri
5.4.99.4 Itaconate competitive Eubacterium barkeri
5.4.99.4 L-malate competitive Eubacterium barkeri
5.4.99.4 mesaconate competitive Eubacterium barkeri
5.4.99.4 succinate competitive Eubacterium barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.1 0.5
-
L-threo-3-methylaspartate
-
Clostridium cochlearium
5.4.99.1 0.5
-
L-threo-3-methylaspartate
-
Clostridium tetanomorphum
5.4.99.1 1.5
-
L-Glu
-
Clostridium cochlearium
5.4.99.1 1.5
-
L-Glu
-
Clostridium tetanomorphum
5.4.99.4 7.1
-
2-Methyleneglutarate
-
Eubacterium barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.4 170000
-
sucrose density gradient centrifugation Eubacterium barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.1 Glu Cereibacter sphaeroides
-
?
-
?
5.4.99.1 Glu Rhodospirillum rubrum
-
?
-
?
5.4.99.1 Glu Clostridium cochlearium
-
?
-
?
5.4.99.1 Glu Clostridium tetanomorphum
-
?
-
?
5.4.99.4 additional information Eubacterium barkeri inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.1 Cereibacter sphaeroides
-
-
-
5.4.99.1 Clostridium cochlearium
-
-
-
5.4.99.1 Clostridium tetanomorphum
-
-
-
5.4.99.1 Rhodospirillum rubrum
-
-
-
5.4.99.4 Eubacterium barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.1 purification of component E and component S Clostridium tetanomorphum
5.4.99.4
-
Eubacterium barkeri

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.99.1 L-threo-3-methylaspartate = L-glutamate mechanism Clostridium tetanomorphum
5.4.99.4 2-Methyleneglutarate = 2-methylene-3-methylsuccinate mechanism Eubacterium barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.4 0.51
-
preparation which is heavily contaminated with methylitaconate isomerase Eubacterium barkeri

Storage Stability

EC Number Storage Stability Organism
5.4.99.4 -80°C, protein concentration 10 mg/ml, about 10% loss of activity after 6 months Eubacterium barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.1 Glu
-
Cereibacter sphaeroides ?
-
?
5.4.99.1 Glu
-
Rhodospirillum rubrum ?
-
?
5.4.99.1 Glu
-
Clostridium cochlearium ?
-
?
5.4.99.1 Glu
-
Clostridium tetanomorphum ?
-
?
5.4.99.1 L-Glu
-
Clostridium cochlearium threo-3-Methylaspartate
-
?
5.4.99.1 L-Glu r Cereibacter sphaeroides threo-3-Methylaspartate
-
?
5.4.99.1 L-Glu r Rhodospirillum rubrum threo-3-Methylaspartate
-
?
5.4.99.1 L-Glu r Clostridium tetanomorphum threo-3-Methylaspartate
-
?
5.4.99.1 L-Glu equilibrium favours glutamate formation Cereibacter sphaeroides threo-3-Methylaspartate
-
?
5.4.99.1 L-Glu equilibrium favours glutamate formation Rhodospirillum rubrum threo-3-Methylaspartate
-
?
5.4.99.1 L-Glu equilibrium favours glutamate formation Clostridium tetanomorphum threo-3-Methylaspartate
-
?
5.4.99.1 L-threo-3-methylaspartate
-
Clostridium cochlearium L-glutamate
-
?
5.4.99.1 L-threo-3-methylaspartate
-
Clostridium tetanomorphum L-glutamate
-
?
5.4.99.4 2-Methyleneglutarate r Eubacterium barkeri 2-Methylene-3-methylsuccinate i.e methylitaconate ?
5.4.99.4 additional information inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate Eubacterium barkeri ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.1 40
-
maximal activity in a 30 min incubation period Clostridium tetanomorphum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.1 55
-
rapidly inactivated Clostridium tetanomorphum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.1 8.5
-
-
Clostridium tetanomorphum

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.99.1 Cobalamin
-
Cereibacter sphaeroides
5.4.99.1 Cobalamin
-
Rhodospirillum rubrum
5.4.99.1 Cobalamin
-
Clostridium cochlearium
5.4.99.1 Cobalamin and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes Clostridium tetanomorphum
5.4.99.4 cobamide required Eubacterium barkeri
5.4.99.4 vitamin B12 dependent on Eubacterium barkeri