Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ducruix, A.; Hounwanou, N.; Reinbolt, J.; Boulanger, Y.; Blanquet, S.
    Purification and reversible subunit dissociation of overproduced Escherichia coli phenylalanyl-tRNA synthetase (1983), Biochim. Biophys. Acta, 741, 244-250.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.20 38000
-
 2 * 98000 (alpha) + 2 * 38000 (beta), SDS-PAGE Escherichia coli
6.1.1.20 98000
-
 2 * 98000 (alpha) + 2 * 38000 (beta), SDS-PAGE Escherichia coli
6.1.1.20 250000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Escherichia coli
-
 overproducing strain carrying a hybrid pBR322 plasmid containing the pheS-pheT locus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.20
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.20 19.8
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 tetramer 2 * 98000 (alpha) + 2 * 38000 (beta), SDS-PAGE Escherichia coli