BRENDA - Enzyme Database show

Characterization of glutamate-1-semialdehyde aminotransferase of Synechococcus. Steady-state kinetic analysis

Smith, M.A.; Kannangara, C.G.; Grimm, B.; von Wettstein, D.; Eur. J. Biochem. 202, 749-757 (1991)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.3.8
expression in Escherichia coli
Synechococcus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
4,5-Dioxovalerate
competitive inhibition of pyridoxamine-phosphate form of the enzyme and mixed-type inhibition of the pyridoxal-phosphate form of the enzyme
Synechococcus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.001
-
4,5-Diaminovalerate
-
Synechococcus sp.
5.4.3.8
0.067
-
(S)-4-Amino-5-oxopentanoate
-
Synechococcus sp.
5.4.3.8
1.4
-
4,5-Dioxovalerate
-
Synechococcus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.3.8
Synechococcus sp.
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
5.4.3.8
L-glutamate 1-semialdehyde = 5-aminolevulinate
ping-pong bi-bi mechanism in which 4,5-diaminovalerate is the second substrate and 4,5-dioxovalerate is an alternative first substrate
Synechococcus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3441
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3441
Synechococcus sp.
-
5.4.3.8
4,5-Diaminovalerate
is a substrate for the pyridoxal 5'-phosphate form of the enzyme
3441
Synechococcus sp.
?
-
-
-
-
5.4.3.8
4,5-Dioxovalerate
is a substrate for the pyridoxamine form of the enzyme
3441
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3441
Synechococcus sp.
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.95
-
(S)-4-Amino-5-oxopentanoate
-
Synechococcus sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxamine 5'-phosphate
the pyridoxal-phosphate form of the enzyme and the pyridoxamine-phosphate form of the enzyme are similarly active
Synechococcus sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
expression in Escherichia coli
Synechococcus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxamine 5'-phosphate
the pyridoxal-phosphate form of the enzyme and the pyridoxamine-phosphate form of the enzyme are similarly active
Synechococcus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
4,5-Dioxovalerate
competitive inhibition of pyridoxamine-phosphate form of the enzyme and mixed-type inhibition of the pyridoxal-phosphate form of the enzyme
Synechococcus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.001
-
4,5-Diaminovalerate
-
Synechococcus sp.
5.4.3.8
0.067
-
(S)-4-Amino-5-oxopentanoate
-
Synechococcus sp.
5.4.3.8
1.4
-
4,5-Dioxovalerate
-
Synechococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3441
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3441
Synechococcus sp.
-
5.4.3.8
4,5-Diaminovalerate
is a substrate for the pyridoxal 5'-phosphate form of the enzyme
3441
Synechococcus sp.
?
-
-
-
-
5.4.3.8
4,5-Dioxovalerate
is a substrate for the pyridoxamine form of the enzyme
3441
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3441
Synechococcus sp.
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.95
-
(S)-4-Amino-5-oxopentanoate
-
Synechococcus sp.