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Literature summary extracted from

  • Blackwell, C.M.; Turner, J.M.
    Microbial metabolism of amino alcohols. Purification and properties of coenzyme B12-dependent ethanolamine ammonia-lyase of Escherichia coli (1978), Biochem. J., 175, 555-563.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.3.1.7 ethanolamine, dithiothreitol, glycerol and KCl protect the apoenzyme from inactivation Escherichia coli
4.3.1.7 K+ stabilizes the enzyme during dialysis or storage Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.7 cyanocobalamin irreversible Escherichia coli
4.3.1.7 DL-1,3-diaminopropan-2-ol competitive Escherichia coli
4.3.1.7 DL-1-aminopropan-2-ol competitive Escherichia coli
4.3.1.7 hydroxycobalamin irreversible; noncompetitive Escherichia coli
4.3.1.7 iodoacetamide in absence of ethanolamine Escherichia coli
4.3.1.7 L-2-Aminopropan-1-ol competitive Escherichia coli
4.3.1.7 methylcobalamin irreversible Escherichia coli
4.3.1.7 p-hydroxymercuribenzoate 0.0001 mM, 50% inhibition; even in the presence of ethanolamine Escherichia coli
4.3.1.7 Urea above 1 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.7 0.03
-
ethanolamine pH 7 Escherichia coli
4.3.1.7 0.3
-
ethanolamine pH 5.8 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.7 K+ not required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.7 560400
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.7 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.7
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.7 13.9
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.3.1.7 -20°C, Tris/HCl buffer supplemented with glycerol, dithiothreitol, KCl and ethanolamine, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.7 ethanolamine
-
Escherichia coli acetaldehyde + NH3
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.7 7 8
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.7 vitamin B12 Km: 0.00042 mM Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.7 0.0001
-
p-hydroxymercuribenzoate
-
Escherichia coli