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Literature summary extracted from

  • Kalghatgi, K.K.; Rao, P.V.S.
    Microbial L-phenylalanine ammonia-lyase. Purification, subunit structure and kinetic properties of the enzyme from Rhizoctonia solani (1975), Biochem. J., 149, 65-72.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 2-mercaptoethanol slight Rhizoctonia solani
4.3.1.24 4-coumarate
-
 Rhizoctonia solani
4.3.1.24 caffeate
-
 Rhizoctonia solani
4.3.1.24 CN- irreversible; NaCN Rhizoctonia solani
4.3.1.24 D-Phe
-
 Rhizoctonia solani
4.3.1.24 dihydrocaffeic acid
-
 Rhizoctonia solani
4.3.1.24 dithiothreitol slight Rhizoctonia solani
4.3.1.24 GSH slight Rhizoctonia solani
4.3.1.24 L-Cys slight Rhizoctonia solani
4.3.1.24 NaBH4 irreversible Rhizoctonia solani
4.3.1.24 NaHSO3 irreversible Rhizoctonia solani
4.3.1.24 p-chloromercuribenzoate slight Rhizoctonia solani
4.3.1.24 phenylpyruvate
-
 Rhizoctonia solani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.24 70000
-
 alpha2,beta2, 2 * 70000 + 2 * 90000, SDS-PAGE Rhizoctonia solani
4.3.1.24 90000
-
 alpha2,beta2, 2 * 70000 + 2 * 90000, SDS-PAGE Rhizoctonia solani
4.3.1.24 330000
-
 gel filtration Rhizoctonia solani
4.3.1.24 340000
-
 density gradient centrifugation Rhizoctonia solani

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Rhizoctonia solani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.24
-
 Rhizoctonia solani

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.24 additional information
-
-
 Rhizoctonia solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 3,4-dihydroxyphenylalanine 29% of the activity with L-Phe Rhizoctonia solani ?
-
 ?
4.3.1.24 fluorophenylalanine p-fluorophenylalanine, 31% of the activity with L-Phe, o-fluorophenylalanine, 14% of the activity with L-Phe Rhizoctonia solani trans-(4-fluoro)cinnamate + NH3
-
 ?
4.3.1.24 L-Phe
-
 Rhizoctonia solani (E)-cinnamate + NH3
-
 ?
4.3.1.24 L-tyrosine m-Tyr, 10% of the activity with L-Phe Rhizoctonia solani p-coumarate + NH3
-
 ?
4.3.1.24 L-tyrosine L-Tyr, 15% of the activity with L-Phe Rhizoctonia solani p-coumarate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.24 tetramer alpha2,beta2, 2 * 70000 + 2 * 90000, SDS-PAGE Rhizoctonia solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.24 44 46
-
 Rhizoctonia solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 8.7 8.9
-
 Rhizoctonia solani