Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Abell, C.W.; Shen, R.S.
    Phenylalanine ammonia-lyase from the yeast Rhodotorula glutinis (1987), Methods Enzymol., 142, 242-249.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.1.25 analysis rapid quantization of Phe and Tyr in plasma and serum from subjects with phenylketonuria Rhodotorula glutinis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.25 4-coumarate
-
 Rhodotorula glutinis
4.3.1.25 Cinnamic acid trans-cinnamic acid Rhodotorula glutinis
4.3.1.25 D-Phe
-
 Rhodotorula glutinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.25 0.15
-
 L-Tyr
-
 Rhodotorula glutinis
4.3.1.25 0.25
-
 L-Phe
-
 Rhodotorula glutinis
4.3.1.25 4.2
-
 trans-cinnamate
-
 Rhodotorula glutinis
4.3.1.25 3170
-
 NH3
-
 Rhodotorula glutinis

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.25 Rhodotorula glutinis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.25
-
 Rhodotorula glutinis

Reaction

EC Number Reaction Comment Organism Reaction ID
4.3.1.25 L-phenylalanine = trans-cinnamate + NH3 ordered uni-bi reaction sequence Rhodotorula glutinis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.25 additional information
-
-
 Rhodotorula glutinis

Storage Stability

EC Number Storage Stability Organism
4.3.1.25 -60°C, enzyme concentration 20-40 mg/ml, stable for 6 months Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.25 L-Phe
-
 Rhodotorula glutinis (E)-cinnamate + NH3
-
 r
4.3.1.25 L-tyrosine L-Tyr Rhodotorula glutinis p-coumarate + NH3
-
 ?
4.3.1.25 trans-cinnamate + NH3
-
 Rhodotorula glutinis L-Phe
-
 r