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Literature summary extracted from

  • Gao, Y.S.; Alvarez, C.; Nelson, D.S.; Sztul, E.
    Molecular cloning, characterization, and dynamics of rat formiminotransferase cyclodeaminase, a Golgi-associated 58-kDa protein (1998), J. Biol. Chem., 273, 33825-33834.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.5 molecular biology bifunctional formiminotransferase cyclodeaminase provides a novel marker to study ER-Golgi dynamics Rattus norvegicus
4.3.1.4 analysis formiminotransferase cyclodeaminase enzyme complex represents a marker with which to explore ER-Golgi dynamics Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.5 cDNA encoding formiminotransferase cyclodeaminase is cloned, characterized and partially sequenced Rattus norvegicus
4.3.1.4 formiminotransferase-cyclodeaminase enzyme complex Rattus norvegicus

General Stability

EC Number General Stability Organism
2.1.2.5 dimeric, tetrameric and octameric complexes are resistant to proteolysis Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.5 proteinase K degradation of bifunctional formiminotransferase cyclodeaminase Rattus norvegicus
2.1.2.5 Urea degradation of bifunctional formiminotransferase cyclodeaminase Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.5 Golgi membrane formiminotransferase cyclodeaminase, 58K, is a peripherically associated Golgi protein, binding is tight but not dependent on presence of intact microtubules, association is likely to be mediated by a protein, dynamic component of the Golgi, a proportion of FTCD molecules cycles between the Golgi and earlier compartments of the secretory pathway Rattus norvegicus 139
-
4.3.1.4 Golgi apparatus Golgi apparatus, formiminotransferase-cyclodeaminase enzyme complex Rattus norvegicus 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.5 58000
-
 2 * 58000, bifunctional formiminotransferase cyclodeaminase exists as dimeric, tetrameric and octameric complexes, SDS-PAGE, a single 35 kDa protein is cross-linked to each dimer, it may play a role in the association of the protein to the Golgi membrane, functional formiminotransferase activity unit of FTCD is a dimer with binding sites for glutamate and folate Rattus norvegicus
2.1.2.5 58000
-
 4 * 58000, bifunctional formiminotransferase cyclodeaminase exists as dimeric, tetrameric and octameric complexes, SDS-PAGE Rattus norvegicus
2.1.2.5 58000
-
 8 * 58000, bifunctional formiminotransferase cyclodeaminase exists as dimeric, tetrameric and octameric complexes, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Rattus norvegicus bifunctional formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmatic surface of the Golgi apparatus in vivo 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Rattus norvegicus metabolic enzyme involved in the conversion of histidine to glutamic acid 5-formiminotetrahydrofolate + L-glutamate
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.5 Rattus norvegicus
-
 Sprague-Dawley rats
-
4.3.1.4 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.5 kidney
-
 Rattus norvegicus
-
2.1.2.5 liver
-
 Rattus norvegicus
-
4.3.1.4 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.5 additional information 58K: formiminotransferase cyclodeaminase, bifunctional enzyme catalyzing two consecutive steps in the modification of tetrahydrofolate to 5,10-methenyl tetrahydrofolate Rattus norvegicus ?
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate bifunctional formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmatic surface of the Golgi apparatus in vivo Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate metabolic enzyme involved in the conversion of histidine to glutamic acid Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Rattus norvegicus 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.5 dimer 2 * 58000, bifunctional formiminotransferase cyclodeaminase exists as dimeric, tetrameric and octameric complexes, SDS-PAGE, a single 35 kDa protein is cross-linked to each dimer, it may play a role in the association of the protein to the Golgi membrane, functional formiminotransferase activity unit of FTCD is a dimer with binding sites for glutamate and folate Rattus norvegicus
2.1.2.5 octamer 8 * 58000, bifunctional formiminotransferase cyclodeaminase exists as dimeric, tetrameric and octameric complexes, SDS-PAGE Rattus norvegicus
2.1.2.5 tetramer 4 * 58000, bifunctional formiminotransferase cyclodeaminase exists as dimeric, tetrameric and octameric complexes, SDS-PAGE Rattus norvegicus
4.3.1.4 octamer 8* 58000, calculation from sequence of cDNA Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.5 tetrahydrofolate
-
 Rattus norvegicus