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Literature summary extracted from
- Metabolism of formiminoglycine. Formiminotetrahydrofolate cyclodeaminase (1967), J. Biol. Chem., 242, 24-31.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.4 | 10-methyltetrahydrofolate | competitive | Clostridium cylindrosporum |  |
| 4.3.1.4 | 2-hydroxy-2-deaminotetrahydrofolate | competitive | Clostridium cylindrosporum | |
| 4.3.1.4 | 4-amino-4-deoxy-10-methylfolate | competitive | Clostridium cylindrosporum | |
| 4.3.1.4 | 4-amino-4-deoxyfolate | competitive | Clostridium cylindrosporum | |
| 4.3.1.4 | 4-amino-4-deoxytetrahydrofolate | competitive | Clostridium cylindrosporum | |
| 4.3.1.4 | tetrahydrofolate | - |
Clostridium acidi-urici | |
| 4.3.1.4 | tetrahydrofolate | competitive | Clostridium cylindrosporum | |
| 4.3.1.4 | tetrahydrofolate | - |
Sus scrofa | |
| 4.3.1.4 | Zn2+ | - |
Clostridium cylindrosporum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.4 | 0.029 | - |
(-)-formiminotetrahydropteroyltri-L-glutamate | - |
Clostridium cylindrosporum | |
| 4.3.1.4 | 0.031 | - |
(-)-formiminotetrahydrofolate | - |
Clostridium cylindrosporum | |
| 4.3.1.4 | 0.04 | - |
(-)-formiminotetrahydropteroyl-L-aspartate | - |
Clostridium cylindrosporum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.4 | 38000 | - |
calculated from Svedberg equation (sedimentation equilibrium?) | Clostridium cylindrosporum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.4 | 5-formiminotetrahydrofolate | Sus scrofa | - |
? | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | Oryctolagus cuniculus | - |
? | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | Clostridium cylindrosporum | - |
? | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | Clostridium acidi-urici | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.4 | Clostridium acidi-urici | - |
- |
- |
| 4.3.1.4 | Clostridium cylindrosporum | - |
- |
- |
| 4.3.1.4 | Oryctolagus cuniculus | - |
- |
- |
| 4.3.1.4 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.4 | - |
Clostridium cylindrosporum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.3.1.4 | liver | - |
Sus scrofa | - |
| 4.3.1.4 | liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.4 | additional information | - |
- |
Sus scrofa |
| 4.3.1.4 | additional information | - |
- |
Clostridium cylindrosporum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.3.1.4 | -20°C, stable for several months at concentrations of 1 mg/ml | Clostridium cylindrosporum |
| 4.3.1.4 | enzyme loses activity after repeated freezing and thawing | Clostridium cylindrosporum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.4 | (-)-formiminotetrahydrofolate | - |
Clostridium cylindrosporum | ? | - |
? | |
| 4.3.1.4 | (-)-formiminotetrahydropteroyl-gamma-L-glutamyl-gamma-L-glutamyl-L-glutamate | - |
Clostridium cylindrosporum | ? | - |
? | |
| 4.3.1.4 | (-)-formiminotetrahydropteroyl-L-aspartate | - |
Clostridium cylindrosporum | ? | - |
? | |
| 4.3.1.4 | (-/+)-formiminotetrahydropteroate | - |
Clostridium cylindrosporum | ? | - |
? | |
| 4.3.1.4 | (-/+)-formiminotetrahydropteroyl-D-glutamate | - |
Clostridium cylindrosporum | ? | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | - |
Sus scrofa | 5,10-methenyltetrahydrofolate + NH3 | - |
r | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | - |
Oryctolagus cuniculus | 5,10-methenyltetrahydrofolate + NH3 | - |
r | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | - |
Clostridium acidi-urici | 5,10-methenyltetrahydrofolate + NH3 | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | reaction is reversible to small extent at pH 8.2 | Clostridium cylindrosporum | 5,10-methenyltetrahydrofolate + NH3 | - |
r | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | - |
Sus scrofa | ? | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | - |
Oryctolagus cuniculus | ? | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | - |
Clostridium cylindrosporum | ? | - |
? | |
| 4.3.1.4 | 5-formiminotetrahydrofolate | - |
Clostridium acidi-urici | ? | - |
? | |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.4 | 25 | 45 | enzyme activity increases in a linear manner, temperature optimum is not determined | Clostridium cylindrosporum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.4 | 7.2 | - |
- |
Clostridium cylindrosporum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.4 | 5 | 8 | pH 5: 20% of maximal activity, pH 8: 30% of maximal activity | Clostridium cylindrosporum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.4 | additional information | - |
- |
Clostridium cylindrosporum |
| 4.3.1.4 | 9.8 | - |
stable for 2 h at 0°C | Clostridium cylindrosporum |
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