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Literature summary extracted from

  • Klee, C.B.
    Metal activation of histidine ammonia-lyase. Metal ion-sulfhydryl group relationship (1972), J. Biol. Chem., 247, 1398-1406.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.3 5,5'-dithiobis(2-nitrobenzoic acid) protection of reduced enzyme by Cd2+ against inactivation Pseudomonas sp.
4.3.1.3 EDTA potent inhibitor of the reduced enzyme; reduced enzyme Pseudomonas sp.
4.3.1.3 additional information
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.3 additional information
-
additional information
-
Pseudomonas sp.
4.3.1.3 6
-
L-histidine oxidized enzyme, in the absence of added metal Pseudomonas sp.
4.3.1.3 6.3
-
L-histidine reduced enzyme in the presence of 0.01 M EDTA Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.3 Cd2+ stimulates oxidized and reduced enzyme Pseudomonas sp.
4.3.1.3 Fe2+ required for optimal activity Pseudomonas sp.
4.3.1.3 Mn2+ stimulates oxidized and reduced enzyme Pseudomonas sp.
4.3.1.3 additional information
-
Pseudomonas sp.
4.3.1.3 Zn2+ required for optimal activity Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.3 Pseudomonas sp.
-
ATCC 11,299b
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.3 additional information
-
-
Pseudomonas sp.
4.3.1.3 1.2
-
0.1 mM Ca2+, oxidized enzyme Pseudomonas sp.
4.3.1.3 2.3
-
0.1 mM Mn2+, oxidized enzyme Pseudomonas sp.
4.3.1.3 2.8
-
0.1 mM Fe2+, oxidized enzyme Pseudomonas sp.
4.3.1.3 4.3
-
0.1 mM Zn2+, oxidized enzyme Pseudomonas sp.
4.3.1.3 6.2
-
0.1 mM Ca2+, reduced enzyme Pseudomonas sp.
4.3.1.3 7
-
0.1 mM Fe2+ or 0.1 mM Zn2+, reduced enzyme Pseudomonas sp.
4.3.1.3 9.1
-
0.1 mM Mn2+, reduced enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.3 L-histidine natural substrate Pseudomonas sp. urocanate + NH3
-
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