BRENDA - Enzyme Database show

Purification and characterization of glutamate 1-semialdehyde aminotransferase from barley expressed in Escherichia coli

Berry-Lowe, S.L.; Grimm, B.; Smith, M.A.; Kannangara, C.G.; Plant Physiol. 99, 1597-1603 (1992)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.3.8
expression in Escherichia coli
Hordeum vulgare
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.025
-
(S)-4-Amino-5-oxopentanoate
-
Hordeum vulgare
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Hordeum vulgare
4,5-diaminovalerate may be an intermediate in the synthesis of 5-amino-4-oxopentanoate
?
-
-
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Hordeum vulgare
enzyme catalyzes the last step in the conversion of Glu to 5-amino-4-oxopentanoate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.3.8
Hordeum vulgare
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.3.8
recombinant enzyme
Hordeum vulgare
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.3.8
0.384
-
-
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3427
Hordeum vulgare
5-Amino-4-oxopentanoate
-
3427
Hordeum vulgare
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
4,5-diaminovalerate may be an intermediate in the synthesis of 5-amino-4-oxopentanoate
3427
Hordeum vulgare
?
-
-
-
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
enzyme catalyzes the last step in the conversion of Glu to 5-amino-4-oxopentanoate
3427
Hordeum vulgare
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.11
-
(S)-4-Amino-5-oxopentanoate
-
Hordeum vulgare
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
expression in Escherichia coli
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.025
-
(S)-4-Amino-5-oxopentanoate
-
Hordeum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Hordeum vulgare
4,5-diaminovalerate may be an intermediate in the synthesis of 5-amino-4-oxopentanoate
?
-
-
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Hordeum vulgare
enzyme catalyzes the last step in the conversion of Glu to 5-amino-4-oxopentanoate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
recombinant enzyme
Hordeum vulgare
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.3.8
0.384
-
-
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3427
Hordeum vulgare
5-Amino-4-oxopentanoate
-
3427
Hordeum vulgare
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
4,5-diaminovalerate may be an intermediate in the synthesis of 5-amino-4-oxopentanoate
3427
Hordeum vulgare
?
-
-
-
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
enzyme catalyzes the last step in the conversion of Glu to 5-amino-4-oxopentanoate
3427
Hordeum vulgare
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.11
-
(S)-4-Amino-5-oxopentanoate
-
Hordeum vulgare