BRENDA - Enzyme Database show

Separation and partial characterization of enzymes catalyzing delta-aminolevulinic acid formation in Synechocystis sp. PCC 6803

Rieble, S.; Beale, S.I.; Arch. Biochem. Biophys. 289, 289-297 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
gabaculine
0.0005 mM, 50% inhibition. The inhibition is reversible up to 1 h after its addition, if the gabaculine is removed by gel filtration, before the enzyme is incubated by gel filtration, before the enzyme is incubated with substrate. Irreversible inhibition is obtained by preincubation of the enzyme at 30°C either for several hours with gabaculine alone or for a few min with both gabaculine and glutamate-1-semialdehyde
Synechococcus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
98000
-
gel filtration
Synechococcus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Synechococcus sp.
one of three enzymes in formation of 5-aminolevulinic acid from Glu via the five-carbon pathway
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.3.8
Synechococcus sp.
-
PCC 6803
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.3.8
0.00009
-
-
Synechococcus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3426
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3426
Synechococcus sp.
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
one of three enzymes in formation of 5-aminolevulinic acid from Glu via the five-carbon pathway
3426
Synechococcus sp.
?
-
-
-
-
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
gabaculine
0.0005 mM, 50% inhibition. The inhibition is reversible up to 1 h after its addition, if the gabaculine is removed by gel filtration, before the enzyme is incubated by gel filtration, before the enzyme is incubated with substrate. Irreversible inhibition is obtained by preincubation of the enzyme at 30°C either for several hours with gabaculine alone or for a few min with both gabaculine and glutamate-1-semialdehyde
Synechococcus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
98000
-
gel filtration
Synechococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Synechococcus sp.
one of three enzymes in formation of 5-aminolevulinic acid from Glu via the five-carbon pathway
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.3.8
0.00009
-
-
Synechococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3426
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3426
Synechococcus sp.
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
one of three enzymes in formation of 5-aminolevulinic acid from Glu via the five-carbon pathway
3426
Synechococcus sp.
?
-
-
-
-