BRENDA - Enzyme Database show

Biosynthesis of DELTA-aminolevulinate in greening barley leaves: glutamate 1-semialdehyde aminotransferase

Kannangara, C.G.; Gough, S.P.; Carlsberg Res. Commun. 43, 185-194 (1978)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
Aminooxyacetate
20 mM, 57% inhibition
Hordeum vulgare
5.4.3.8
cycloserine
20 mM, 43% inhibition
Hordeum vulgare
5.4.3.8
D-penicillamine
20 mM, 9% inhibition
Hordeum vulgare
5.4.3.8
glyoxylate
20 mM, 27% inhibition
Hordeum vulgare
5.4.3.8
additional information
no inhibition by 3-hydroxyglutamate
Hordeum vulgare
5.4.3.8
p-chloromercuribenzoate
0.1 mM, 45% inhibition
Hordeum vulgare
5.4.3.8
pyridoxal 5'-phosphate
5 mM, 47% inhibition
Hordeum vulgare
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.4
-
(S)-4-Amino-5-oxopentanoate
-
Hordeum vulgare
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.3.8
chloroplast
mature
Hordeum vulgare
9507
-
5.4.3.8
plastid
low specific activity in etiolated plastids compared to greening plastids
Hordeum vulgare
9536
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
67000
-
x * 67000, SDS-PAGE
Hordeum vulgare
5.4.3.8
500000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Hordeum vulgare
the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.3.8
Hordeum vulgare
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.3.8
partial
Hordeum vulgare
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.4.3.8
leaf
greening
Hordeum vulgare
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3419
Hordeum vulgare
5-Amino-4-oxopentanoate
-
3419
Hordeum vulgare
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids
3419
Hordeum vulgare
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
5.4.3.8
?
x * 67000, SDS-PAGE
Hordeum vulgare
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.4.3.8
30
-
-
Hordeum vulgare
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5.4.3.8
14
30
14°C: 41% of maximal activity, 22°C: 76.4% of maximal activity
Hordeum vulgare
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.3.8
8
-
broad
Hordeum vulgare
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.3.8
6.7
8.8
pH 6.7: about 45% of maximal activity, pH 8.8: about 90% of maximal activity
Hordeum vulgare
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
Aminooxyacetate
20 mM, 57% inhibition
Hordeum vulgare
5.4.3.8
cycloserine
20 mM, 43% inhibition
Hordeum vulgare
5.4.3.8
D-penicillamine
20 mM, 9% inhibition
Hordeum vulgare
5.4.3.8
glyoxylate
20 mM, 27% inhibition
Hordeum vulgare
5.4.3.8
additional information
no inhibition by 3-hydroxyglutamate
Hordeum vulgare
5.4.3.8
p-chloromercuribenzoate
0.1 mM, 45% inhibition
Hordeum vulgare
5.4.3.8
pyridoxal 5'-phosphate
5 mM, 47% inhibition
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.4
-
(S)-4-Amino-5-oxopentanoate
-
Hordeum vulgare
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.3.8
chloroplast
mature
Hordeum vulgare
9507
-
5.4.3.8
plastid
low specific activity in etiolated plastids compared to greening plastids
Hordeum vulgare
9536
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
67000
-
x * 67000, SDS-PAGE
Hordeum vulgare
5.4.3.8
500000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Hordeum vulgare
the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
partial
Hordeum vulgare
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.4.3.8
leaf
greening
Hordeum vulgare
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3419
Hordeum vulgare
5-Amino-4-oxopentanoate
-
3419
Hordeum vulgare
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids
3419
Hordeum vulgare
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.3.8
?
x * 67000, SDS-PAGE
Hordeum vulgare
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.4.3.8
30
-
-
Hordeum vulgare
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5.4.3.8
14
30
14°C: 41% of maximal activity, 22°C: 76.4% of maximal activity
Hordeum vulgare
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.3.8
8
-
broad
Hordeum vulgare
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.3.8
6.7
8.8
pH 6.7: about 45% of maximal activity, pH 8.8: about 90% of maximal activity
Hordeum vulgare