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Literature summary extracted from

  • Sato, M.; Kaji, A.
    Exopolygalacturonate lyase produced by Streptomyces massasporeus (1980), Agric. Biol. Chem., 44, 717-721.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.9 EDTA
-
Streptomyces massasporeus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.9 Ca2+ required, optimal concentration is 0.5 mM Streptomyces massasporeus
4.2.2.9 Mn2+ 50% of the stimulation with Ca2+ Streptomyces massasporeus
4.2.2.9 Sr2+ 50% of the stimulation with Ca2+ Streptomyces massasporeus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.9 54000
-
gel filtration Streptomyces massasporeus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.9 Streptomyces massasporeus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.9
-
Streptomyces massasporeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.9 hexagalacturonic acid 51% of the activity with low molecular polygalacturonic acid Streptomyces massasporeus ?
-
?
4.2.2.9 pentagalacturonic acid 60% of the activity with low molecular polygalacturonic acid Streptomyces massasporeus ?
-
?
4.2.2.9 polygalacturonate low molecular polygalacturonic acid, very low activity with high molecular polygalacturonic acid Streptomyces massasporeus unsaturated digalacturonate
-
?
4.2.2.9 tetragalacturonic acid 52% of the activity with low molecular polygalacturonic acid Streptomyces massasporeus ?
-
?
4.2.2.9 trigalacturonic acid 248% of the activity with low molecular polygalacturonic acid Streptomyces massasporeus ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.9 monomer 1 * 54000, SDS-PAGE Streptomyces massasporeus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.9 40
-
-
Streptomyces massasporeus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.9 50
-
pH 7.0, 10 min, stable below Streptomyces massasporeus
4.2.2.9 60
-
pH 7.0, 10 min, 85% loss of activity Streptomyces massasporeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.9 9.5
-
-
Streptomyces massasporeus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.9 3 10 2°C, 72 h, relatively stable Streptomyces massasporeus